ATPases and GTPases catalyze the decomposition of ATP or GTP into ADP or GDP and freephosphate ion. These enzymes play key roles in transport, signal transduction, protein biosynthesis and cell differentiation.Intended Use:Determination of ATPase and GTPase activity.Drug Discovery: high-throughput screen for ATPase or GTPase inhibitors.Sensitivity:Detection of as little of 2pmoles of free phosphate.Test Principle:This ATPase/GTPase BioAssay Kit offers a highly sensitive method for determiningATPase/GTPase activities in a microplate format. Its proprietary formulation features a single reagent for accurate determination of enzyme activity in 30 min at RT. The improved malachite green reagent forms a stable dark green color with liberated phosphate, which is measured on a plate reader (600-660nm).Kit Components:Reagent: 1x50mlAssay Buffer: 1x10mlStandard: 1mM phosphate, 1x1mlStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
