- 中文名称
GAL4 Reporter, BioAssay Kit (Glucocorticoid Receptor Pathway) (Glucocorticoid Receptor, GR, Nuclear Receptor Subfamily 3 Group C Member 1, NR3C1, GAL4/UAS)
- 英文名字
- GAL4 Reporter, BioAssay Kit (Glucocorticoid Receptor Pathway) (Glucocorticoid Receptor, GR, Nuclear Receptor Subfamily 3 Group C Member 1, NR3C1, GAL4/UAS)
- 供应商
- USBiological
- 产品货号
- 298409
- 产品报价
- ￥1/500Tests
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- The glucocorticoid signaling pathway plays an important role in development, fluid homeostasis, cognition, immune response and metabolism. Glucocorticoids are a class of steroid hormones that bind to the glucocorticoid receptor, causing it to translocate to the nucleus. Upon translocation, the receptor can regulate the transcription of a large number of genes, including those that regulate glucose metabolism and inflammatory responses.Sample Type:Cultured cellsIntended Use:Applications include monitoring glucocorticoid signaling pathway activity and for screening activators or inhibitors of the glucocorticoid signaling pathway.Test Principle:The Glucocorticoid Receptor Pathway Reporter BioAssay Kit is designed for monitoring the activity of the glucocorticoid signaling pathway in cultured cells. The kit contains a transfection-ready expression vector for the glucocorticoid receptor ligand binding domain that is fused to the DNA binding domain (DBD) of GAL4 (GAL4 DBD-GR). This fusion construct activates firefly luciferase expression under the control of a multimerized GAL4 upstream activation sequence (UAS). This allows for specific detection of glucocorticoid-induced activation of the glucocorticoid receptor without the need for individual transcriptional targets and with low crossreactivity for other nuclear receptor pathways. The GAL4/UAS reporter is premixed with constitutively expressing Renilla (sea pansy) luciferase vector, which serves as an internal positive control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical for determining pathway-specific effects and background luciferase activity.Kit Components:Reporter (Component A), GAL4/UAS luciferase reporter vector + constitutively expressingRenilla luciferase vector, 1x500ul, (60ng DNA/ul)Negative Control Reporter (Component B), Non-inducible luciferase vector + constitutively expressing Renilla luciferase vector, 1x500ul, (60ng DNA/ul)GAL4 DBD-GR (Component C), Expression vector for ligand binding domain of theglucocorticoid receptor + GAL4 DNA binding domain, 1x250ul, (100ng DNA/ul)Negative Control Expression vector (Component D), Expression vector with GAL4 DNA binding domain only, 1x250ul (100ng DNA/ul)These vectors are ready for transient transfection. They are NOT SUITABLE for transformationand amplification in bacteria.Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对GAL4 Reporter, BioAssay Kit (Glucocorticoid Receptor Pathway) (Glucocorticoid Receptor, GR, Nuclear Receptor Subfamily 3 Group C Member 1, NR3C1, GAL4/UAS)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到GAL4 Reporter, BioAssay Kit (Glucocorticoid Receptor Pathway) (Glucocorticoid Receptor, GR, Nuclear Receptor Subfamily 3 Group C Member 1, NR3C1, GAL4/UAS)产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
-
该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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