Karl Fischer reagent. Imidazole can be used for the elution of histidine containing proteins from divalent cation resins. Imidazole can be used to prepare buffers in the pH range of 6.2-7.8 at 25°C. It is also a chelator for the binding of various divalent cations.Synonyms:1,3-Diazo-2,4-cyclopentadiene; 1H-Imidazole; 1,3-Diazacyclopenta-2,4-diene; 1,3-diazoleCAS No:288-32-4Molecular Formula:C3H4N2Molecular Weight:68.08Divalent Cation Log Binding Constant:Ca2+0.1Mn2+1.6Fe2+3.3Co2+2.4Ni2+2.9Cu2+4.2Zn2+2.0Appearance:Whtie to off-white crystalline powderPurity:≥99%Solubility:Freely soluble in waterMelting Point:~90°CIron (Fe):≤ 0.001%Water (Karl Fisher):≤ 0.2%pH:9.5-11.0 (5% solution at 25°C)Residue after Igniton:≤ 0.1%Storage:Store at 4°CMeets or exceeds ACS specifications
