- 中文名称
Vascular Endothelial Cell Growth Factor, BioAssay ELISA Kit (Mouse) (VEGF, MVCD1, VEGF-A, VPF, Vascular Endothelial Growth Factor Isoform VEGF165, Vascular Permeability Factor)
- 英文名字
- Vascular Endothelial Cell Growth Factor, BioAssay ELISA Kit (Mouse) (VEGF, MVCD1, VEGF-A, VPF, Vascular Endothelial Growth Factor Isoform VEGF165, Vascular Permeability Factor)
- 供应商
- USBiological
- 产品货号
- 228014
- 产品报价
- ￥1/48Tests ￥1/96Tests ￥1/24Tests
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms.Sample Type:Serum, plasma, cell culture supernates and tissue homogenatesIntended Use:For the quantitative determination of mouse vascular endothelial cell growth factor (VEGF) concentrations in serum, plasma, cell culture supernates and tissue homogenates. Sensitivity:0.857pg/mlRange:3.906-250pg/mlSpecificity:This assay has high sensitivity and excellent specificity for detection of mouse VEGF. No significant cross-reactivity or interference between mouse VEGF and analogues was observed.Test Principle:This BioAssay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for VEGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VEGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VEGF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VEGF bound in the initial step. The color development is stopped and the intensity of the color is measured. Kit Components:Assay plate (12x8 coated Microwells), 1x96 wellsStandard (Freeze dried), 2x vials Biotin-antibody (100X), 1x120ul HRP-avidin (100X), 1x120ul Biotin-antibody Diluent, 1x15ml HRP-avidin Diluent, 1x15ml Sample Diluent, 1x50ml Wash Buffer (25X), 1x20ml TMB Substrate, 1x10ml Stop Solution, 1x10ml Adhesive Strip (For 96 wells), 4x strips Instruction manual Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Vascular Endothelial Cell Growth Factor, BioAssay ELISA Kit (Mouse) (VEGF, MVCD1, VEGF-A, VPF, Vascular Endothelial Growth Factor Isoform VEGF165, Vascular Permeability Factor)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Vascular Endothelial Cell Growth Factor, BioAssay ELISA Kit (Mouse) (VEGF, MVCD1, VEGF-A, VPF, Vascular Endothelial Growth Factor Isoform VEGF165, Vascular Permeability Factor)产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
-
该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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