- 中文名称
Soluble Cluster of Differentiation 14, BioAssay ELISA Kit (Human) (sCD14, CD14 Antigen, Monocyte Differentiation Antigen CD14)
- 英文名字
- Soluble Cluster of Differentiation 14, BioAssay ELISA Kit (Human) (sCD14, CD14 Antigen, Monocyte Differentiation Antigen CD14)
- 供应商
- USBiological
- 产品货号
- 227920
- 产品报价
- ￥1/48Tests ￥1/96Tests ￥1/24Tests
- 产品说明书
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- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- CD14 is a surface protein preferentially expressed on monocytes/macrophages. It binds lipopolysaccharide binding protein and recently has been shown to bind apoptotic cells. Alternative splicing results in multiple transcript variants encoding the same isoform.Soluble Cluster of Differentiation 14, BioAssay ELISA Kit is a sandwich ELISA intended for the quantitative determination of sCD14 in human serum, plasma, cell culture supernates, tissue homogenates and urine.Detection Range:0.234-15ng/ml Intra-assay Precision: %CV <8 Inter-assay Precision: %CV <10Sensitivity:The minimum detectable dose of human sCD14 is typically less than 0.229ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.Specificity:This assay has high sensitivity and excellent specificity for detection of human sCD14. No significant crossreactivity or interference between human sCD14 and analogs was observed.Note: Limited by current skills and knowledge, it is impossible for us to complete the crossreactivity detection between human sCD14 and all the analogs. Therefore, crossreaction may still exist.Kit Components:227920A: Microtiter Strips, 1x48 wells or 1x96 wells. Pre-coated; ready to use. 227920B: Standard, 2x1vial227920C: sCD14 (Biotin), 100X, 1x60ul or 1x120ul227920D: Avidin (HRP), 100X, 1x60ul or 1x120ul227920E: Biotin Diluent, 1x7.5ml or 1x15ml227920F: Avidin (HRP) Diluent, 1x7.5ml or 1x15ml227920G: Sample Diluent, 1x25ml or 1x50ml227920H: Wash Buffer, 25X, 1x10ml or 1x20ml227920J: TMB Substrate, 1x5ml or 1x10ml227920K: Stop Solution, 1x5ml or 1x10mlNote: The Stop Solution (227920K) provided with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.Storage and Stability:Store kit at 4°C. The unopended kit is stable for 6 months after receipt. Once opened, the kit is stable up to 4 weeks at 4°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.Assay Summary:1. Prepare reagents, standards and samples as instructed.2. Add 100ul standard or sample to each well. Incubate for 2 hours at 37°C3. Remove the liquid of each well do not wash.4. Add 100ul sCD14 (Biotin), 1X to each well. Incubate for 1 hour at 37°C5. Aspirate and wash 3 times.6. Add 100ul Avidin (HRP), 1X to each well. Incubate for 1 hour at 37°C7. Aspirate and wash 5 times.8. Add 90ul TMB substrate to each well Incubate for 15-30 minutes at 37°C. Protect from light9. Add 50ul Stop Solution to each well. Read at 450nm within 5 minutes.
- 产品特点
- 针对Soluble Cluster of Differentiation 14, BioAssay ELISA Kit (Human) (sCD14, CD14 Antigen, Monocyte Differentiation Antigen CD14)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Soluble Cluster of Differentiation 14, BioAssay ELISA Kit (Human) (sCD14, CD14 Antigen, Monocyte Differentiation Antigen CD14)产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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