- 中文名称
Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase (RuBisCO), BioAssay ELISA Kit (Plant)
- 英文名字
- Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase (RuBisCO), BioAssay ELISA Kit (Plant)
- 供应商
- USBiological
- 产品货号
- 227904
- 产品报价
- ￥1/48Tests ￥1/96Tests ￥1/24Tests
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Ribulose-1,5-bisphosphate carboxylase oxygenase, most commonly known by the shorter name RuBisCO, is an enzyme involved in the Calvin cycle that catalyzes the first major step of carbon fixation, a process by which the atoms of atmospheric carbon dioxide are made available to organisms in the form of energy-rich molecules such as glucose. RuBisCO catalyzes either the carboxylation or the oxygenation of ribulose-1,5-bisphosphate (also known as RuBP) with carbon dioxide or oxygen. RuBisCO is very important in terms of biological impact because it catalyzes the primary chemical reaction by which inorganic carbon permanently enters the biosphere. Many autotrophic bacteria and archaea fix carbon via the reductive acetyl CoA pathway, the 3-hydroxypropionate cycle or the reverse Krebs cycle, but they make up a relatively minor portion of global net primary production. Phosphoenolpyruvate carboxylase PEPC only temporarily fixes carbon. RuBisCO is also the most abundant protein in leaves, and is considered to be the most abundant protein on Earth.It accounts for 50% of soluble leaf protein in C3 plants (20-30% of total leaf nitrogen) and 30% of soluble leaf protein in C4 plants (5-9% of total leaf nitrogen).Given its important role in the biosphere, there are currently efforts to genetically engineer crop plants so as to contain more efficient RuBisCO.Sample Type:Plant tissuesIntended Use:For the quantitative determination of endogenic ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) concentrations in plant tissues. Sensitivity:1.56ug/mlRange:3.12-800ug/mlSpecificity:This assay has high sensitivity and excellent specificity for detection of plant RuBisCO. No significant cross-reactivity or interference between plant RuBisCO and analogues was observed.Test Principle:This BioAssay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with antigen. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for RuBisCO. The competitive inhibition reaction is launched between with pre-coated RuBisCO and RuBisCO in samples with the antibody. Then add a Horseradish Peroxidase (HRP) conjugated goat-anti-rabbit IgG antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of RuBisCO in the sample. The color development is stopped and the intensity of the color is measured. Kit Components:Assay plate, 1x96 wellsStandard, 2x500ul Antibody (100X), 1x60ul HRP-conjugate (100X), 1x120ul Antibody Diluent, 1x10ml HRP-conjugate Diluent, 1x20ml Sample Diluent, 2x20ml Sample Extraction Buffer (25X), 1x20ml Wash Buffer (25X), 1x20ml TMB Substrate, 1x10ml Stop Solution, 1x10ml Adhesive Strip (For 96 wells), 4x strips Instruction manual Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase (RuBisCO), BioAssay ELISA Kit (Plant)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase (RuBisCO), BioAssay ELISA Kit (Plant)产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
-
- 注意
-
该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
-
