- 中文名称
Monocyte Chemotactic Protein 1 (MCP-1) BioAssay ELISA Kit (Human)
- 英文名字
- Monocyte Chemotactic Protein 1 (MCP-1) BioAssay ELISA Kit (Human)
- 供应商
- USBiological
- 产品货号
- 227814
- 产品报价
- ￥1/48Tests ￥1/96Tests ￥1/24Tests
- 产品说明书
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- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. This protein is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.Intended Use:The Monocyte Chemotactic Protein 1 (MCP-1) BioAssay ELISA Kit (Human) BioAssay ELISA Kit is a sandwich ELISA intended for the quantitative determination of MCP-1 concentration in human serum, plasma, tissue homogenate, cerebrospinal fluid, saliva, urine, ascitic fluid and cell culture supernatant. Sensitivity:<30.184pg/mlDetection Range:31.25-2000pg/mlPrecision:Intra-assay CV: <8%Inter-assay CV: <10%Specificity:This assay has high sensitivity and excellent specificity for detection of human MCP-1. No significant cross-reactivity or interference between human MCP-1 and analogues was observed.Test Principle:This BioAssay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for MCP-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MCP-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MCP-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MCP-1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Principle of the Assay:This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MCP-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MCP-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MCP-1 is added to the wells. After washing, avidin-conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MCP-1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Kit Components:227814A: Microtiter Strips, 12x8 Microwell Strips. Pre-coated; ready to use. 227814B: Standard (lyophilized), 2 vials227814C: Biotin-Antibody (100x Concentrate), 1x120ul227814D: HRP-Avidin, (100x Concentrate), 1x120ul227814E: Biotin-Antibody Diluent, 1x15ml227814F: HRP-Avidin Diluent, 1x15ml227814G: Sample Diluent, 1x50ml227814H: Wash Buffer (25X Concentrate), 1x20ml227814J: TMB Substrate, 1x10ml227814K: Stop Solution, 1x10mlStorage and Stability:Store all kit components at 4°C. Unopened kit is stable for 6 months after receipt. Once opened, the kit is stable for 1 month provided this is within the expiration date of the kit.Opened Kit: Coated plate may be stored at 4°C for up to 1 month, sealed in the aluminum foil bag. Standard, Biotin-Antibody and HRP-Avidin may be stored at 4°C or -20°C for up to 1 month. The rest of the components may be stored at 4°C for up to 1 month.For maximum recovery of contents, centrifuge the original vials after thawing and prior to removing the cap.Assay Summary:1. Prepare reagents, standards and samples as instructed.2. Add 100ul standard or sample to each well. Incubate for 2 hours at 37°C3. Remove the liquid of each wee; do not wash.4. Add 100ul Biotin-Antibody (1x) to each well. Incubate for 1 hour at 37°C5. Aspirate and wash 3 times.6. Add 100ul HRP-Avidin (1x) to each well. Incubate for 1 hour at 37°C7. Aspirate and wash 5 times.8. Add 90ul TMB substrate to each well Incubate for 15-30 minutes at 37°C. Protect from light9. Add 50ul Stop Solution to each well. Read at 450nm within 5 minutes.
- 产品特点
- 针对Monocyte Chemotactic Protein 1 (MCP-1) BioAssay ELISA Kit (Human)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Monocyte Chemotactic Protein 1 (MCP-1) BioAssay ELISA Kit (Human)产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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