- 中文名称
Equine IgG (Immunoglobulin G) BioAssay ELISA Kit
- 英文名字
- Equine IgG (Immunoglobulin G) BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- 227732
- 产品报价
- ￥1/48Tests ￥1/96Tests ￥1/24Tests
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- The Equine IgG (Immunoglobulin G) BioAssay ELISA Kit is a competitive ELISA intended for the quantitative determination of IgG concentration in equine serum and plasma.Detection Range:25-400ug/ml Sensitivity:<23.764ug/mlPrecision: Intra-Assay CV: <8%Inter-Assay CV: <10%Principle of the Assay:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate has been pre-coated with equine IgG. Standards, samples and HRP-conjugated anti-equine IgG are added to the appropriate microtiter plate wells and a competitive reaction is launched between plate-coated equine IgG and equine IgG in the standards or samples for limited antibody binding sites on the HRP-conjugated anti-equine IgG reagent. The wells are washed to remove unbound materials. TMB substrate solution is then added to the wells; color development is stopped and absorbance read at 450nm wavelength. The intensity of the color is inversely proportional to the amount of equine IgG in the samples.Kit Components:227665A: Microtiter Strips, 1x96 wells. Pre-coated; ready to use. 227665B: Standard, 0ug/ml, 1x500ul227665C: Standard, 25ug/ml, 1x500ul227665D: Standard, 50ug/ml, 1x500ul227665E: Standard, 100ug/ml, 1x500ul227665F: Standard, 200ug/ml, 1x500ul227665G: Standard, 400ug/ml, 1x500ul227665H: HRP Conjugate, 1x6ml227665J: Sample Diluent 2x20ml227665K: Wash Buffer, 25X, 1x20ml227665L: TMB Substrate, 1x10ml227665M: Stop Solution, 1x10mlNote: The Stop Solution (227732K) provided with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.Storage and Stability:Store kit at 4°C. The unopended kit is stable for 6 months after receipt. Once opened, the kit is stable up to 4 weeks at 4°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.Assay Summary:1. Prepare reagents and samples as instructed.2. Set a Blank well without any solution3. Add 50ul Standard or sample to each well.4. Add 50ul HRP Conjugate to each well (do not add any to the blank well).5. Incubate for 30 minutes at 37°C.6. Aspirate and wash 5 times.7. Add 90ul TMB Substrate to each well.8. Incubate for 20 minutes at 37°C. Protect from light9. Add 50ul Stop Solution to each well. Read at 450nm within 5 minutes.
- 产品特点
- 针对Equine IgG (Immunoglobulin G) BioAssay ELISA Kit 该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Equine IgG (Immunoglobulin G) BioAssay ELISA Kit 产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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