- 中文名称
Biotin, BioAssay ELISA Kit
- 英文名字
- Biotin, BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- 227542
- 产品报价
- ￥1/48Tests ￥1/96Tests ￥1/24Tests
- 产品说明书
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- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Biotin, BioAssay ELISA Kit is a competitive ELISA intended for the quantitative determination of Biotin in human and mouse serum and plasma.Detection Range:12.5-200pg/mlSensitivity:<4.986pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest Biotin concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.Intra-assay Precision: %CV <8 Inter-assay Precision: %CV <10Specificity:This assay has high sensitivity and excellent specificity for detection of Biotin. No significant cross-reactivity or interference between biotin and analogues was observed.Test Principle:This BioAssay employs the direct competitive inhibition enzyme immunoassay technique. The microplate was pre-coated with Avidin. Add Biotin (Standards or samples) to the well, and then add HRP-conjugated Biotin. A competitive inhibition reaction is launched between Biotin (Standards or samples) and HRP conjugated Biotin with the Avidin. The substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of Biotin bound in the initial step. The color development is stopped and the intensity of the color is measured. Kit Components:227542A: Microtiter Strips, 1x48 wells or 1x96 wells*227542B: Standard, 200pg/ml, 1x100ul or 1x200ul*227542C: HRP Conjugate, 100X, 1x30ul or 1x60ul227542D: HRP Diluent, 1x10ml or 1x20ml227542E: Sample Diluent, 1x20ml or 1x40ml227542F: Wash Buffer, 25X, 1x20ml227542G: TMB Substrate, 1x10ml or 1x20ml227542H: Stop Solution, 1x10ml or 1x20mlNote: The Stop Solution (227542H) provided with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.Storage and Stability:Store *227542B and *227542C at -20ºC. Store all other kit components at 4°C. Stable for 6 months after receipt. Once opened, the kit is stable for 1 month providing this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.Assay Summary:1. Prepare reagents and samples as instructed.2. Set a Blank well without any solution3. Add 50ul Standard or sample to each well.4. Add 50ul HRP Conjugate, 1X to each well (Not to the Blank well).5. Incubate for 40 minutes at 37°C.6. Aspirate and wash 5 times.7. Add 90ul TMB Substrate to each well.8. Incubate for 20 minutes at 37°C. Protect from light.9. Add 50ul Stop Solution to each well. Read at 450nm within 10 minutes.
- 产品特点
- 针对Biotin, BioAssay ELISA Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Biotin, BioAssay ELISA Kit产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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