Fluorogenic tri-peptide substrate for measuring the trypsin-like peptidase activity of the 20S proteasome. It is cleaved exclusively at the amide R-AMC bond to release fluorescent product. Shows low Km and high specific activity. This substrate is useful for inhibitor screening and kinetic analysis.Excitation: Excitation: 380nm. Emission: 460nm.Molecular Formula:C30H46N10O6Molecular Weight:642.8Sequence: Acetyl-Arg-Leu-Arg-7-amido-4-methylcoumarin Solubility:Soluble in DMSO, methanol or water (aqueous solutions).Storage and Stability:Stable for at least 1 year after receipt when stored at -20°C.