- 中文名称
ACES Signal Sequence BioAssay Kit
- 英文名字
- ACES Signal Sequence BioAssay Kit
- 供应商
- USBiological
- 产品货号
- 226803
- 产品报价
- ￥1/1Kit
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- E. coli is often the host strain of first choice for the production of recombinant proteins. Despite its long and successful history, several limitations remain when the desired protein cannot be expressed in a functional state. This is often due to the inherent properties of expressingheterologous proteins in the cytoplasm. One means of overcoming these impediments is to express the protein such that it is secreted into an environment that is more conducive to correct folding and where proteolytic degradation is minimized, such as the periplasm or extracellular matrix. Periplasmic or extracellular protein export has been exploited for the production of a number of recombinant proteins. The extracellular accumulation of a target protein provides several advantages. These include: A Simplified Downstream Purification Scheme, Enhanced Biological Activity, Higher Product Stability and Solubility.Protein secretion in E. coli and other Gram-negative bacteria is an extensively reviewed subject.1 It is also a very complex process. The pAES30 series vectors utilize signal sequences that represent the three translocation pathways of the type II secretion process of E. coli.The type II secretion mechanism is a two-step process. Protein translocation through the inner membrane is accomplished by way of one of three pathways: SecB-dependent (SEC), signal recognition particle (SRP) or twin-arginine translocation (TAT) (Fig. 1). Most proteins in E. coli are exported to the periplasm via one of these pathways with the larger majority using SEC. Extracellular secretion by a type II mechanism is via the main terminal branch pathway, which involves a poorly defi ned complex of 12-16 proteins that are not expressed under normal laboratory conditions.2 Therefore, most of the protein exported by the type II systems remains resident in the periplasm, which is also where most secreted recombinant proteins accumulate.3Test Principle:Six signal sequences representing the three translocation pathways of E. coli (SRP, SEC, TAT) are fused to the N-terminus of the Target Protein facilitating its secretion to the periplasmic space. Translocation through the inner membrane causes removal of the signal sequence resulting in a Target Protein with its native N-terminus. Once in the periplasmic space, the Target Protein can then be recovered by using any number of reagents that perforate the outer cell membrane, causing release of the Target Protein into the culture medium. Alternatively, cells can be harvested by centrifugation and a cold osmotic shock protocol used to extract the recombinant Target Protein from the periplasmic space.Kit Components:pAES30, 1x10ugpAES31, 1x10ugpAES32, 1x10ugpAES33, 1x10ugpAES34, 1x10ugpAES35, 1x10ugJM109 Competent Cells, 2x200ul LB Broth (Miller) 1x1L MixTurbo Broth 1x1L MixSuperior Broth 1x1L MixPower Broth 1x1L MixHyper Broth 1x1L MixGlucose M9Y 1x1L MixGlucose Nutrient Mix, 1x21gAugmedium 25ml mixInducer Solution A, 1x 1mlInducer Solution B, 1x100mlSecretion Enhancer Solution A 1x60mlSecretion Enhancer Solution B, 1x60mlStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对ACES Signal Sequence BioAssay Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到ACES Signal Sequence BioAssay Kit产品后将其置于-20°C/-70°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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