- 中文名称
TNF alpha, BioAssay ELISA Kit (Tumor Necrosis Factor Alpha) (Rat)
- 英文名字
- TNF alpha, BioAssay ELISA Kit (Tumor Necrosis Factor Alpha) (Rat)
- 供应商
- USBiological
- 产品货号
- 220336
- 产品报价
- ￥1/100Tests
- 产品说明书
- 点击查看
- 购买方式
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- The rat TNF alpha BioAssay ELISA Kit is based on the standard sandwich enzyme-linked immunosorbent assay technology. Sample Type:Serum and plasma (heparin, EDTA). Cell culture supernatants.Intended Use:For use in quantitative protein detection, establishing normal range.Sensitivity:<1pg/mlRange:15.6-1000pg/ml (cell culture supernates), 7.8-500pg/ml (rat serum, plasma)Specificity:Recognizes rat TNF alpha. This ELISA kit shows no cross-reactivity with other relevant proteins.Test Principle:This assay employs a monoclonal antibody specific for rat TNF alpha coated on a 96-well plate. Standards and test samples are added to the wells and TNF alpha present in a sample is bound to the wells by the immobilized antibody. A biotinylated detection polyclonal antibody from goat specific for TNF alpha is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, Avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the rat TNF alpha captured onto the plate. Kit Components:TNF alpha Microplate coated with anti-rat monoclonal Ab against TNF alpha, 1x96 wells
Lyophilized recombinant rat TNF alpha standard (10ng/vial), 2x vials
Biotinylated anti-rat TNF alpha antibody, 1x130ul
Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul
Sample diluent buffer, 1x30ml
Antibody diluent buffer, 1x12ml
ABC diluent buffer, 1x12ml
TMB color developing agent (Colorless), 1x10ml
TMB stop solution, 1x10ml Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对TNF alpha, BioAssay ELISA Kit (Tumor Necrosis Factor Alpha) (Rat)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到TNF alpha, BioAssay ELISA Kit (Tumor Necrosis Factor Alpha) (Rat)产品后将其置于-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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