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细胞分析试剂盒
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中文名称

Succinyl-CoA Synthatase Activity Colorimetric BioAssay Kit (SCS, Succinyl-CoA Ligase, Succinate Thiokinase)

英文名字
Succinyl-CoA Synthatase Activity Colorimetric BioAssay Kit (SCS, Succinyl-CoA Ligase, Succinate Thiokinase)
供应商
USBiological
产品货号
220289
产品报价
¥1/100Tests
产品说明书
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产品新闻
背景资料
USBiological品牌是全球有名的抗原抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理,是行业中少有的致力于服务客户,帮助客户,且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应,专业的产品和解决方案支持,稳健快捷的交货周期,优质放心的售后服务。我们致力于为您提供有价值的产品和服务,在意您的成功!
产品描述
Succinyl-CoA Synthetase (SCS, also called Succinyl-CoA ligase, Succinate Thiokinase) (EC 6.2.1.5) is a critical enzyme in the citric acid cycle and an important metabolic intermediate for porphyrin, heme and ketone body biosynthesis. It is located in the mitochondrial matrix and is a heterodimer composed of one alpha and one beta subunit. In humans, Succinyl-CoA Synthetase deficiency causes the build-up of lactic acid leading to lactic acidosis, which can be fatal in infants. Measurement and analysis of SCS activity is useful for both mechanistic studies as well as for diagnostic purposes. Sample Type:Animal tissues: heart, liver, muscle, etc. Cell culture: adherent or suspension cells. Purified mitochondria.Intended Use:For use in the measurement of Succinyl-CoA Synthetase activity in various tissues/cells and for analysis of the cell signaling pathway.Sensitivity:This kit can detect <0.1mU of Succinyl-CoA Synthetase activity in a variety of samples.Test Principle:In the Succinyl-CoA Synthetase Activity BioAssay, SCS converts succinate into succinyl-CoA in the presence of ATP and CoA. Succinyl-CoA reacts with the Developer to form a colored product with strong absorbance at 450nm. This assay kit is simple, sensitive, and high-throughput adaptable. Kit Components:SCS Assay Buffer, 1x25ml 
SCS Substrate Mix, lyophilized, 1x vial 
SCS Enzyme Mix, lyophilized, 1x vial 
SCS Developer, lyophilized, 1x vial 
NADH Standard, lyophilized, 1x vial SCS Positive Control, lyophilized, 1x vial Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
产品特点
针对Succinyl-CoA Synthatase Activity Colorimetric BioAssay Kit (SCS, Succinyl-CoA Ligase, Succinate Thiokinase)该产品的特点优势,欢迎查阅官网提供的产品说明书。
保存建议
建议收到Succinyl-CoA Synthatase Activity Colorimetric BioAssay Kit (SCS, Succinyl-CoA Ligase, Succinate Thiokinase)产品后将其置于4°C/-20°C/-70°C保存。
其他
Usbiological公司是美国著名的抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品,为科研用户提供了诸多超值选择。
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