- 中文名称
Sphingomyelinase, Activity Colorimetric BioAssay Kit (SMase)
- 英文名字
- Sphingomyelinase, Activity Colorimetric BioAssay Kit (SMase)
- 供应商
- USBiological
- 产品货号
- 220277
- 产品报价
- ￥1/100Tests
- 产品说明书
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- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Sphingomyelinase (SMase) cleaves sphingomyelin to produce phosphocholine and ceramide. The activation of SMase leads to increased production of ceramide, which acts as a lipid second messenger that induces a variety of cell regulatory phenomena such as apoptosis, cell differentiation, cell proliferation, and sterol homeostasis. There are five distinct types of SMases.Sample Type:Animal tissues: brain, heart, kidney, etc. Cell culture: Adherent or suspension cells.Intended Use:For use in the measurement of SMase activity in various tissues/cells extracts and screening of SMase inhibitors.Sensitivity:This high-throughput adaptable assay kit can detect sphingomyelinase activity as low as 1.25mU/ml (~5 uU/mg tissue/cells) in a variety of samples.Test Principle:The SMase Activity BioAssay Kit provides a simple and sensitive method for measuring neutral-SMase enzymatic activity using colorimetry (OD 570nm). Neutral sphingomyelinase (N-SMase) is a Mg2+ sensitive enzyme that can be activated by a host of physiologically relevant and structurally diverse molecules. In this assay, SMase converts sphingomyelin substrate to phosphocholine and ceramide, which is then further utilized to produce an intermediate that reacts with a highly specific probe to generate color. Kit Components:SMase Assay Buffer, 1x28ml SMase Probe, DMSO, 1x200ul SMase Substrate, lyophilized, 1x vial SMase Enzyme Mix I, lyophilized, 1x vialSMase Enzyme Mix II, lyophilized, 1x vialCholine Standard, lyophilized, 1x vialSMase Positive Control, lyophilized, 1x vialSMase Extraction Detergent, 1x1mlStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Sphingomyelinase, Activity Colorimetric BioAssay Kit (SMase) 该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Sphingomyelinase, Activity Colorimetric BioAssay Kit (SMase) 产品后将其置于-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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