- 中文名称
Pyruvate Dehydrogenase Activity Colorimetric BioAssay Kit (PDH)
- 英文名字
- Pyruvate Dehydrogenase Activity Colorimetric BioAssay Kit (PDH)
- 供应商
- USBiological
- 产品货号
- 220212
- 产品报价
- ￥1/100Tests
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Pyruvate Dehydrogenase (PDH) (EC 1.2.4.1) has a vital role in carbohydrate metabolism. It forms a well-characterized enzyme complex with dihydrolipoyl transacetylase (E2) and dihydrolipoyl dehydrogenase (E3). PDH converts pyruvate into acetyl-CoA in the presence of NAD and CoA, and links glycolysis to the citric acid cycle. PDH activity is inhibited by high intracellular ratios of ATP/ADP, NADH/NAD or Acetyl-CoA/CoA. In humans, PDH deficiency reduces mitochondrial function and is linked to neurodegenerative diseases. PDH deficiency is X-linked; it results in 2 forms of abnormality: a metabolic form (lactic acidosis) and a neurological form (seizure and/or neuropathological spasm). Recent studies show that PDH is a target of oncogene-induced senescence; activation of PDH enhances pyruvate utilization and increases respiration and redox stress.Sample Type:Animal tissues: heart, liver, muscle, etc. Cell culture: Adherent or suspension cells. Purified mitochondria.Intended Use:For use in the measurement of pyruvate dehydrogenase activity in various tissues/cells and for analysis of the cell signaling pathway.Sensitivity:Can measure pyruvate dehydrogenase activity lower than 0.1mU in a variety of samples.Test Principle:This PDH BioAssay kit provides a quick and easy way for monitoring PDH activity in various samples. In the assay, PDH converts pyruvate into an intermediate, which reduces the developer to a colored product with strong absorbance at 450nm. Kit Components:PDH Assay Buffer, 1x25mlPDH Substrate, lyophilized, 1x vial PDH Developer, lyophilized, 1x vial NADH Standard, lyophilized, 1x vial PDH Positive Control, 1x10ulStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Pyruvate Dehydrogenase Activity Colorimetric BioAssay Kit (PDH)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Pyruvate Dehydrogenase Activity Colorimetric BioAssay Kit (PDH)产品后将其置于-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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