- 中文名称
Malate Dehydrogenase Activity Colorimetric BioAssay Kit (MDH)
- 英文名字
- Malate Dehydrogenase Activity Colorimetric BioAssay Kit (MDH)
- 供应商
- USBiological
- 产品货号
- 220018
- 产品报价
- ￥1/100Tests
- 产品说明书
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- 购买方式
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Malate Dehydrogenase (MDH) (EC 1.1.1.37) is an important enzyme which reversibly converts L-malate into oxaloacetate in the presence of NAD. In eukaryotic cells, malate dehydrogenase has 2 isoforms: MDH1 and MDH2. MDH1 is cytosolic and participates in the malate-aspartate shuttle, which transports malate into mitochondria for utilization in ATP generation whereas MDH2 is a mitochondrial enzyme and part of the citric acid cycle. MDH activity is increased in some neurodegenerative diseases such as Alzheimer’s disease, and abnormal MDH activity in serum can serve as a diagnostic tool for severe liver damage (e.g. Hepatocellular carcinoma).Sample Type:Animal tissues such as liver, heart, muscle, etc. Cell culture: Adherent or suspension cells. Mitochondria.Intended Use:For use in the measurement of malate dehydrogenase activity in various tissues/cells and for analysis of the citric acid cycle and malate-asparate shuttle.Sensitivity:The assay can detect Malate Dehydrogenase (MDH) Activity <0.5mU in various sample types.Test Principle:In the Malate Dehydrogenase Activity BioAssay kit, MDH reacts with malate to form an intermediate. The generated intermediate reacts with MDH Developer to form a colored product with strong absorbance at 450nm. Kit Components:MDH Assay Buffer, 1x20ml MDH Substrate, lyophilized, 1x vial MDH Enzyme Mix, lyophilized, 1x vial MDH Developer, lyophilized, 1x vial NADH Standard, lyophilized, 1x vial MDH Positive Control, lyophilized, 1x vial Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Malate Dehydrogenase Activity Colorimetric BioAssay Kit (MDH)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Malate Dehydrogenase Activity Colorimetric BioAssay Kit (MDH)产品后将其置于-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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