- 中文名称
Lipolysis, 3T3-L1, Colorimetric BioAssay Kit
- 英文名字
- Lipolysis, 3T3-L1, Colorimetric BioAssay Kit
- 供应商
- USBiological
- 产品货号
- 220010
- 产品报价
- ￥1/100Tests
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Lipolysis is the hydrolysis of triglycerides within the cell into glycerol and free fatty acids. The glycerol and free fatty acids are then released into the bloodstream or culture media. Lipolysis occurs in essentially all cells, but is most abundant in white and brown adipose tissue. Deficiencies in lipolysis lead to increased intracellular lipid accumulation, resulting in abnormal cellular physiology, hyperlipidemia, and insulin resistance. Lipolysis can be induced by catecholamines and certain hormones.Sample Type:Primary adipocytes. Cell culture: 3T3-L1 cells.Intended Use:Measurement of lipolysis in 3T3-L1 cells or adipocytes. Screening compounds that influence lipolysis, mechanistic studies and studies on metabolic dysfunctions.Sensitivity:This assay kit can detect <200pmol of glycerol.Test Principle:This kit includes synthetic catecholamines, Isoproterenol, which activates beta-adrenergic receptors. This leads to activation of adenylate cyclase, which catalyzes the conversion of ATP to cAMP. cAMP then serves as a second messenger to activate hormone- sensitive lipase, which hydrolyzes the triglycerides. This pathway can be inhibited by insulin. This 3T3-L1 Lipolysis BioAssay kit is simple and easy-to-use. The assay measures glycerol released from 3T3-L1 cells after induction of lipolysis using colorimetric method. The color intensity is directly proportional to the amount of glycerol. Kit Components:Lipolysis Assay Buffer, 1x17ml Lipolysis Wash Buffer, 1x22mlGlycerol Assay Buffer, 1x25mlGlycerol Probe, in DMSO, Anhydrous, 1x0.2ml Glycerol Enzyme Mix, lyophilized, 1x vial Glycerol Standard, 100mM, 1x0.2ml Isoproterenol, 10mM, 1x50ulStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Lipolysis, 3T3-L1, Colorimetric BioAssay Kit 该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Lipolysis, 3T3-L1, Colorimetric BioAssay Kit 产品后将其置于-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
-
- 注意
-
该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
-
