Jacalin is isolated from Jackfruit seeds and purified by affinity chromatography. It is a glycoprotein with two non-covalently bound subunits (14.6 and 17.1kD) and belongs to the family of galactose-binding lectins. Jacalin is preferably used in applications to isolate IgA from human serum, isolating human plasma glycoproteins and for applications in histochemistry. Jacalin is supplied as a white to light-yellow lyophilized powder without additives. The purity of the lectin is determined by SDS-PAGE, generating two homogeneous bands at 14kD and 17kD. Source:Jackfruit seeds from Artocarpus heterophyllus Molecular Weight: ~14.6kD (b chain) and ~17.1kD (full protein)Endotoxin:<0.1EU/mgAA Sequence:MAYSSLLSLS VLALLFSISS ADTRKWFLAN GINQNPIGII EAAVGVSEDL LNLNGMEAKN NKQSGKSQTV IVGPWGAQVS TSSNGKAFDD GAFTGIREIN LSYNKETAIG DFQVIYDLNG SPYVGQNHTS FIKGFTPVKI SLDFPSEYIV DVSGYTGKVS GYVVVRSLTF TNKKTYGPY GVTSGTPFNL PIENGLVVGF KGSIGYWLDY FSMYLSLStorage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
产品形式
该产品为冻干粉形式。 No preservative added,缓冲液为:sterile ddH2O。