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中文名称

IGF-1, BioAssay ELISA Kit (Insulin-like Growth Factor-1) (Human)

英文名字
IGF-1, BioAssay ELISA Kit (Insulin-like Growth Factor-1) (Human)
供应商
USBiological
产品货号
219871
产品报价
¥1/100Tests
产品说明书
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产品新闻
背景资料
USBiological品牌是全球有名的抗原抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理,是行业中少有的致力于服务客户,帮助客户,且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应,专业的产品和解决方案支持,稳健快捷的交货周期,优质放心的售后服务。我们致力于为您提供有价值的产品和服务,在意您的成功!
产品描述
The human IGF-I BioAssay ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IGF-I.Sample Type:Serum, plasma, cell culture supernatants and urine
Intended Use:Applications include: quantitative protein detection, establishing normal range and validation of antibody array results.Sensitivity:The minimum detectable concentration of IGF-1 is <0.1ng/ml. Range:0.1-30ng/mlSpecificity:Recognizes human IGF-I. This ELISA kit only can detect a free form of human IGF-I and shows no cross-reactivity with any of the cytokines tested e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IL-1a, IL-1b, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-g, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1a, MIP-1b, MIP-1d, PARC, RANTES, SCF, TARC, TGF-b, TIMP-1, TIMP-2, TNF-a, TNF-b, TPO, VEGF.Test Principle:This BioAssay employs an antibody specific for human IGF-I coated on a 96-well plate. Standards and samples are pipetted into the wells and IGF-I present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IGF-I antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGF-I bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450nm. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.Kit Components:IGF-I Microplate (Item A), 1x96wellsWash Buffer Concentrate, 20X, (Item B), 1x25mlStandards (Item C), 2x vialsAssay Diluent C (Item L), 2x30mlDetection Antibody IGF-I (Item F), 2x vialHRP-Streptavidin Concentrate (Item G), 120X, 1x200ul TMB One-Step Substrate Reagent (Item H), 1x12ml Stop Solution (Item I), 1x8mlStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
产品特点
针对IGF-1, BioAssay ELISA Kit (Insulin-like Growth Factor-1) (Human)该产品的特点优势,欢迎查阅官网提供的产品说明书。
保存建议
建议收到IGF-1, BioAssay ELISA Kit (Insulin-like Growth Factor-1) (Human)产品后将其置于-20°C保存。
其他
Usbiological公司是美国著名的抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品,为科研用户提供了诸多超值选择。
注意
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