- 中文名称
HDAC3 Immunoprecipitation (IP) and Activity BioAssay Kit (Histone Deacetylase 3)
- 英文名字
- HDAC3 Immunoprecipitation (IP) and Activity BioAssay Kit (Histone Deacetylase 3)
- 供应商
- USBiological
- 产品货号
- 219833
- 产品报价
- ￥1/25Tests
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Histone deacetylases (HDACs) play a central role in controlling cell cycle regulation, cell differentiation, and tissue development. These proteins have crucial roles in development and physiology, especially in the heart and nervous system. They are also deeply involved in cellular proliferation, cell cycle and apoptosis. HDAC3 is primarily localized in the nucleus, but can also be found in the cytoplasm and at the plasma membrane. Sample Type:Human, mouse or rat. Cell lysate and nuclear extract.Intended Use:Applications include: Immunoprecipitation of HDAC3 complex from cell & nuclear extract(s). Measurement of HDAC3 activity of immunoprecipitated complex/purified enzyme and screening for activators or inhibitors of HDAC3.Test Principle:The HDAC3 IP & Activity BioAssay Kit provides an antibody-based method to specifically immunoprecipitate the HDAC3 complex from cells & tissues and to measure HDAC3 activity fluorometrically. HDA3 is immunoprecipitated from cell or nuclear extract(s) using HDAC3 antibody followed by capturing the complex on protein-A/G beads. The immunoprecipitated complex is incubated with the HDAC substrate. Only the deacetylated substrate is cleaved by the Developer to produce a fluorophore, which can be easily analyzed using a fluorescence plate reader.Kit Components:HDAC Assay Buffer, 1x9mlExtraction Buffer, 1x100mlHDAC Substrate, 1x100ulDeveloper, 1x500ulAMC (7-amino-4-methyl coumarin) Standard, 1mM, 1x100ulRabbit HDAC3 Antibody, 1x500ulRabbit IgG (Control Antibody), 1x250ulProtein-A/G Sepharose Beads (50% slurry in 20% ethanol/H2O), 1x650ulPositive Control (Jurkat Cell Lysate), lyophilized, 1x vialUnit Definition: One unit of HDAC is the amount of enzyme that generates one nanomole of deacetylated substrate/min/mg at 37°C.Detection method:Fluorescence (Ex/Em=380/500nm)Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对HDAC3 Immunoprecipitation (IP) and Activity BioAssay Kit (Histone Deacetylase 3)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到HDAC3 Immunoprecipitation (IP) and Activity BioAssay Kit (Histone Deacetylase 3)产品后将其置于-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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