- 中文名称
HDAC1 Immunoprecipitation (IP) and Activity BioAssay Kit (Histone Deacetylase 1)
- 英文名字
- HDAC1 Immunoprecipitation (IP) and Activity BioAssay Kit (Histone Deacetylase 1)
- 供应商
- USBiological
- 产品货号
- 219832
- 产品报价
- ￥1/25Tests
- 产品说明书
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- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Histone deacetylases (HDACs) play a central role in controlling cell cycle regulation, cell differentiation, and tissue development. HDAC1 is ubiquitously expressed and plays a role in cellular senescence, cardiomyocyte differentiation and hypertrophy, aging of the liver, myelination and adult neurogenesis to name a few functions. Sample Type:Human, mouse or rat. Cell lysate, nuclear extract and tissue extract.Intended Use:Applications include: Immunoprecipitation of HDAC1 complex from cell, nuclear, or tissue extract(s). Measurement of HDAC1 activity of immunoprecipitated complex/purified enzyme and screening for activators or inhibitors of HDAC1.Test Principle:The HDAC1 IP & Activity BioAssay Kit provides an antibody-based method to specifically immunoprecipitate the HDAC1 complex from cells & tissues and to measure HDAC1 activity fluorometrically. HDAC1 is immunoprecipitated from cell, nuclear or tissue extract(s) using HDAC1 antibody followed by capturing the complex on protein-A/G beads. The immunoprecipitated complex is incubated with the HDAC substrate. Only the deacetylated substrate is cleaved by the Developer to produce a fluorophore, which can be easily analyzed using a fluorescence plate reader.Kit Components:HDAC Assay Buffer, 1x9ml
Cell Lysis Buffer, 1x100ml
HDAC Substrate, 1x100ul
Developer, 1x500ul
AFC (7-amino-4-trifluoromethyl coumarin) Standard, 1mM, 1x100ul
Rabbit HDAC1 Antibody, 1x150ul
Rabbit IgG (Control Antibody), 1x75ul
Protein-A/G Sepharose Beads (50% slurry in 20% ethanol/H2O), 1x650ulPositive Control (Jurkat Cell Lysate), lyophilized, 1x vialUnit Definition:One unit of HDAC is the amount of enzyme that generates one nanomole of deacetylated substrate/min/mg at 37°C. Detection method: Fluorescence (Ex/Em=380/500nm) Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对HDAC1 Immunoprecipitation (IP) and Activity BioAssay Kit (Histone Deacetylase 1)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到HDAC1 Immunoprecipitation (IP) and Activity BioAssay Kit (Histone Deacetylase 1)产品后将其置于-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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