The Caspase-5 Colorimetric BioAssay Kit provides a simple and convenient means for assaying the activity of caspase-5 and related caspases that recognize the sequence WEHD.Sample Type:Cell and tissue lysates Intended Use:For use in detecting the early/middle stages of apoptosis and differentiating apoptosis from necrosis.Specificity:Recognizes Caspase-5. Species Crossreactivity: mammalianTest Principle:The assay is based on spectrophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate WEHD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm. Comparison of the absorbance of pNA from a treated sample with an untreated control allows determination of the fold increase in Caspase-5 activity.Kit Components:Cell Lysis Buffer, 1x100ml 2X Reaction Buffer, 4x2ml WEHD-pNA, 4mM, 1x0.5ml DTT, 1M, 1x0.4ml Dilution Buffer, 1x100mlStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
