Sample Type:Live cellsIntended Use:For use in detecting the early/middle stages of apoptosis and differentiating apoptosis from necrosis.Specificity:Recognizes Annexin V. Species Crossreactivity: mammalian Test Principle:The Annexin V-PE-Cy5 Apoptosis Detection BioAssay Kit is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate of Annexin V, a protein that has a strong natural affinity for PS. The one-step staining procedure takes only 10 minutes. In addition, the assay can be directly performed on live cells, without the need for fixation. Results can be analyzed by flow cytometry or fluorescence microscopy.Kit Components:Annexin V-PE-Cy5, 1x125ul 1X Binding Buffer, 1x12.5mlStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
产品特点
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保存建议
建议收到Annexin V (PE-Cy5) Apoptosis Detection BioAssay Kit产品后将其置于4°C保存。