- 中文名称
alpha-Ketoglutarate Dehydrogenase Activity Colorimetric BioAssay Kit (alpha-KGDH)
- 英文名字
- alpha-Ketoglutarate Dehydrogenase Activity Colorimetric BioAssay Kit (alpha-KGDH)
- 供应商
- USBiological
- 产品货号
- 219370
- 产品报价
- ￥1/100Tests
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Alpha-Ketoglutarate Dehydrogenase (Alpha-KGDH) (EC 1.2.4.2) is a key enzyme in the citric acid cycle. It forms an enzyme complex with dihydrolipoamide succinyl transferase (E2) and dihydrolipoamide dehydrogenase (E3). Alpha-KGDH converts alpha-ketoglutarate into succinyl-CoA in the presence of NAD and CoA. It is highly regulated by intracellular ATP/ADP and NADH/NAD ratios and calcium. In humans, decreased KGDH activity can lead to neurodegenerative diseases such as Alzheimer’s disease. Recent studies show that alpha-KGDH is a target of oxidative stress; reactive oxygen species (ROS) inhibit KGDH activity which diminishes its critical function and can cause a bioenergetic deficit. Sample Type:Animal tissues: liver, heart, muscle, etc. Cell culture: Adherent or suspension cellsPurified mitochondriaIntended Use:For use in the measurement of alpha-Ketoglutarate dehydrogenase activity in various tissues/cells and analysis of cell signaling pathways such as the citric acid cycle, lysine degradation or tryptophan metabolism in various cell types.Test Principle:The alpha-KGDH BioAssay kit provides a quick and easy way for monitoring alpha-KGDH activity in various samples. In the assay, alpha-KGDH converts alpha-ketoglutarate into an intermediate which reduces the probe to a colored product with strong absorbance at 450nm. The assay is simple, sensitive and can detect alpha-ketoglutarate dehydrogenase activity lower than 0.1mU in a variety of samples.Kit Components:KGDH Assay Buffer, 1x25ml KGDH Substrate, lyophilized, 1x vial KGDH Developer, lyophilized, 1x vialNADH Standard, lyophilized, 1x vialKGDH Positive Control, 1x50ulStorage and Stability:Store kit at -20°C, protected from light. Unopened kit is stable for 6 months at -20°C. Briefly centrifuge small vials prior to opening.
- 产品特点
- 针对alpha-Ketoglutarate Dehydrogenase Activity Colorimetric BioAssay Kit (alpha-KGDH)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到alpha-Ketoglutarate Dehydrogenase Activity Colorimetric BioAssay Kit (alpha-KGDH)产品后将其置于-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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