中文名称

HAT Medium Supplement (Hypoxanthine-Aminopterin-Thymidine), 50X (Powder)

英文名字

HAT Medium Supplement (Hypoxanthine-Aminopterin-Thymidine), 50X (Powder)

供应商

USBiological

产品货号

216061

产品报价

￥1/10x10ml

产品说明书

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购买方式

银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868

产品新闻

背景资料

USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！

产品描述

The production of monoclonal antibodies involves the fusion of myeloma cells with spleen cells and the selection of hybridomas (cell hybrids) in culture utilizing hypoxanthine-aminopterin-thymidine (HAT) supplemented medium. Aminopterin blocks the synthesis of DNA by inhibiting dihydrofolate reductase Cells that lack the ability to utilize the salvage pathway for nucleotide synthesis are eliminated. Cells that possess hypoxanthine-guanine phosphoribosyl transferase (HPRTase) and thymidine kinase (TK) enzymes can utilize the salvage pathway if supplied with hypoxanthine and thymidine. The purpose of the medium is to: (1) selectively kill unfused myeloma cells that are well adapted to tissue culture and would otherwise outgrow any hybridomas produced and (2) eliminate any myeloma-myeloma hybridomas that lack HPRTase. HPRTase positive spleen-spleen hybridomas, although not sensitive to aminopterin, are normally short-lived in culture. After selection is complete (~10-14 weeks), aminopterin is diluted from the culture by several passages of the cells in hypoxanthine-thymidine (HT) supplemented medium (~2-3 weeks) before transfer into normal hybridoma growth medium.Form: White to off-white powderSolubility (in 10ml water): Colorless to faint yellow, clear liquidSterility:Sterile (gamma-irradiated)Endotoxin:≤100EU/mlComponents:Each vial contains:Hypoxanthin: 6.8mgAminopterin: 0.088mgThymidine: 1.94mgPreparation Instructions:Reconstitute vial to 10ml with sterile water to yield a 50X solution containing 0.005M hypoxanthine, 0.00005M aminopterin and 0.0008M thymidine. When 10ml of 50X concentrate are diluted to 500ml with sterile tissue culture medium, the final concentrations of hypoxanthine, aminopterin and thymidine are 100uM, 0.4uM and 16uM, respectively.Storage and Stability:Store lyophilized product at -20°C prior to reconstitution. Lyophilized powder is stable for 6 months at 20°C after receipt. Upon reconstitution to 10ml, the concentrated stock can be stored at -20°C.

产品形式

艾美捷向您提供的HAT Medium Supplement (Hypoxanthine-Aminopterin-Thymidine), 50X (Powder)产品形式查看产品说明书，建议按照产品说明书进行应用操作。

保存建议

接收到HAT Medium Supplement (Hypoxanthine-Aminopterin-Thymidine), 50X (Powder)产品后，置于-20°C温度下保存。

其他

Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。

注意

该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。

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