- 中文名称
TNF-b, Human, BioAssay ELISA Kit (Tumor Necrosis Factor beta)
- 英文名字
- TNF-b, Human, BioAssay ELISA Kit (Tumor Necrosis Factor beta)
- 供应商
- USBiological
- 产品货号
- 216059
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- TNF-beta (TNF-b) is a protein of 171 amino acids in length and N-glycosylated at position 62. Some cell lines secrete varying glycosylated forms of the factor that may differ also in their biological activities. The protein does not contain disulfide bonds and forms heteromers with LT-beta (Lymphotoxin-beta) that anchors the complexes in the membrane. TNF-b and TNF-a show ~30 percent sequence homology and bind to the same receptor, and murine and human TNF-bare highly homologous at 74 percent. The biological functions of human TNF-b(hTNF-b) include promoting lymphoid development, enhancing normal host resistance to infection, and inhibiting growth of malignant tumors. Elevated hTNF-b levels are usually related to Crohn's Disease, multiple sclerosis, juvenile rheumatoid adrenoleucodystrophy, hypercalcemia of adult T cell leukemia, arthritis, bulbous pemphigoid, non-Hodgkin's lymphoma, and pregnancy. TNF-b is cytolytic or cytostatic for many tumor cells and acts as a mitogen for B-lymphocytes. TNF-b is also a chemo-attractant for neutrophils, increases phagocytosis, and increases adhesion to the endothelium. TNF-b promotes the proliferation of fibroblasts and is likely involved in the wound healing processes in vivo. TNF-b not only induces the synthesis of GM-CSF, G-CSF, IL-1, but also acts as an anti-angiogenesis factor. Hemorrhagic necrosis of tumors induced by TNF-b in vivo is likely the result of an inhibition of the growth of endothelial cells. The TNF-b gene has a length of ~3kb and contains four exons. The gene maps to human chromosome 6p23-6q12, which is ~1.2kb apart from the TNF-a gene. The results obtained from the study of the TNF-b gene polymorphisms suggest that the gene may modify individual susceptibility to breast cancer in women.Intended Use:This Human TNF-b ELISA kit is to be used for the in vitro quantitative determination of human Tumor Necrosis Factor Beta (TNF-b) concentrations in serum, plasma, and cell culture supernatant.Sensitivity:<11pg/mlRange:11-8000pg/mlTest Principle:This TNF-β enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to TNF-b. Standards or samples are then added to the appropriate microtiter plate wells and incubated. After washing to remove unbound TNF-b and other components of the sample, biotin-conjugated polyclonal antibody specific to TNF-b is added and incubated. If present, TNF-b will bind and become immobilized by the antibody pre-coated on the wells and then become “sandwiched” by the biotin conjugate. In order to quantitatively determine the amount of TNF-b present in the sample, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Avidin is a tetramer containing four identical subunits, each having a high affinity-binding site for biotin. The wells are thoroughly washed to remove all unbound HRP-conjugated Avidin and a TMB (3,3'5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain TNF-b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.This kit includes two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant testing) in order to measure the concentration of TNF-b in the samples. According to the testing system, the standard provided is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D) versus TNF-b concentration (pg/ml). The concentration of TNF-b in the samples is then determined by comparing the O.D. of the samples to the standard curve.Kit Components:TNf-b MICROTITER PLATE
BIOTIN CONJUGATE
AVIDIN CONJUGATE
TNf-b STANDARD
SERUM ASSAY BUFFER
CALIBRATOR DILUENT I
CALIBRATOR DILUENT II
WASH BUFFER (20X/96 wells, 30X/192 wells)
SUBSTRATE A
SUBSTRATE B
STOP SOLUTIONStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对TNF-b, Human, BioAssay ELISA Kit (Tumor Necrosis Factor beta)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到TNF-b, Human, BioAssay ELISA Kit (Tumor Necrosis Factor beta)产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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