- 中文名称
TNF-a, mouse, BioAssay ELISA Kit (Tumor Necrosis Factor alpha)
- 英文名字
- TNF-a, mouse, BioAssay ELISA Kit (Tumor Necrosis Factor alpha)
- 供应商
- USBiological
- 产品货号
- 216058
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Tumor Necrosis Factor alpha (TNF-a) is a multifunctional proinflammatory cytokine, mainly secreted by activated macrophages. The cytokine was named for its remarkable ability to cause hemorrhagic necrosis of tumors in mice. It is implicated with a variety of biological procedures including systemic inflammation, cell proliferation, apoptosis, lipid metabolism, and coagulation. It is well documented that TNF-a functions through its receptors, TNFR1 (p55) and TNFR2 (p75). TNF-a plays an important role in the immune response to bacterial, and certain fungal, viral, parasitic invasions, in tissue remodeling, autoimmune-diseases and the necrosis of specific tumors. The pleiotropic effect of TNF-a regulation is attributed to its ability to trigger multiple signaling pathways simultaneously. TNF-a hyper-expression in response to the components of some bacteria such as LPS can cause life threatening septic shock. Recombinant TNF-a, in combination with chemotherapy, has been applied for synergistic treatment of soft sarcomas, melanomas and other irresectable tumors. Anti-TNF-a therapy has been used for treatment of rheumatoid arthritis.Intended Use:This Mouse TNF-alpha (a) ELISA Kit is to be used for the in vitro quantitative determination of mouse tumor necrosis factor alpha (mouse TNF-a) concentrations in serum and cell culture supernatant.Sensitivity:<4.5pg/mlRange:15.6-1000pg/mlTest Principle:This mouse TNF-a enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to mouse TNF-a. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated monoclonal antibody preparation specific for mouse TNF-a and incubated. Mouse TNF-a if present, will bind and become immobilized by the antibody pre-coated on the wells and then be “sandwiched” by biotin conjugate. The microtiter plate wells are thoroughly washed to remove unbound mouse TNF-a and other components of the sample. In order to quantify the amount of mouse TNF-a present in the sample, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Avidin is a tetramer containing four identical subunits that each has a high affinity-binding site for biotin. The wells are thoroughly washed to remove all unbound HRP-conjugated Avidin and a TMB (3,3'5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain mouse TNF-a, biotin-conjugated antibody, and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.In order to measure the concentration of mouse TNF-a in the samples, this kit includes two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant testing.) According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D) versus mouse TNF-a concentration (pg/ml). The concentration of mouse TNF-a in the samples is then determined by comparing the O.D. of the samples to the standard curve.Kit Components:MOUSE TNf-a MICROTITER PLATE
BIOTIN CONJUGATE
AVIDIN CONJUGATE
MOUSE TNF-a STANDARD
CALIBRATOR DILUENT I
CALIBRATOR DILUENT II
WASH BUFFER (20X/96 wells, 30X/192 wells)
SUBSTRATE A
SUBSTRATE B
STOP SOLUTIONStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对TNF-a, mouse, BioAssay ELISA Kit (Tumor Necrosis Factor alpha)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到TNF-a, mouse, BioAssay ELISA Kit (Tumor Necrosis Factor alpha)产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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