- 中文名称
Interleukin-17A, Mouse, BioAssay ELISA Kit (IL-17A)
- 英文名字
- Interleukin-17A, Mouse, BioAssay ELISA Kit (IL-17A)
- 供应商
- USBiological
- 产品货号
- 216008
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Mouse Interleukin-17A (mIL-17A), also know as CTLA-8, is a secreted, homodimeric glycoprotein linked by disulfide link with a molecular mass of about 35kD. The cytokine is the proto-type of a newly discovered pro-inflammatory cytokine family which consists of IL-17A, IL-17B, IL-17C, IL-17D, IL-17E (IL-25) and IL-17F. IL-17A is mainly produced by a group of CD4 T helper cells termed Th17 cells. In addition to Th17 cells, CD8 T helper cells, natural killer cells and granulocytes, can also secret the cytokine after proper stimulation. IL17A exhibits structural homology and cross-species bioactivity between human and mouse cells. IL-17A acts through its receptor IL-17R. It was found to stimulate the expression of pro-inflammatory cytokines including IL-6, MCP-1, G-CSF,GM-CSF,IL-1alpha and TNF-alpha.Most of those cytokines plays a role in the proliferation, maturation and chemotaxis of neutrophils. Elevated levels of IL-17A have been found to implicate with autoimmune diseases, airway inflammation, allograft rejection, inflammatory bowel disease, psoriasis, cancer, vascular inflammation and development of atherosis. This mouse IL-17A ELISA is a 3.5-hour solid phase immunoassay readily applicable to measure mouse IL-17A levels in cell culture supernatant and mouse serum in the range of 0 to 1000pg/ml. It showed no cross reactivity with other cytokines tested. This mouse IL-17A ELISA is expected to be effectively used for further investigations into the relationship between IL-17A and the various conditions mentioned. Intended Use:This mouse Interleukin-17A ELISA Kit is designed for the in vitro quantitative determination of mouse interleukin-17A (IL-17A) concentrations in cell culture supernatant and mouse serum. Sensitivity:<2pg/mlRange:15.6-1000pg/mlTest Principle:This mouse IL-17A enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to mouse IL-17A. Standards or samples are then added to the appropriate microtiter plate wells. A biotin-conjugated antibody preparation specific for mouse IL-17A was added and incubated. Mouse IL-17A, if present, will bind and become immobilized by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed to remove unbound mouse IL-17A and other components in the sample. Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Avidin is a tetramer containing four identical subunits that each has a high affinity-binding site for biotin. The wells are thoroughly washed to remove all unbound HRP-conjugated Avidin, and aTMB(3,3'5, 5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain mouse IL-17A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 2nm. In order to measure the concentration of mouse IL-17A in the samples this kit includes two calibration diluents (Calibrator Diluent I for serum testing and Calibrator Diluent II for cell culture supernatant testing.) According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D) versus mouse IL-17A concentration (pg/ml). The concentration of IL-17A in the samples is then determined by comparing the O.D. of the samples to the standard curve.Kit Components:ANTI-MOUSE IL-17A MICROTITER PLATE
BIOTIN CONJUGATE
AVIDIN CONJUGATE
MOUSE IL-17 STANDARD
CALIBRATOR DILUENT I
CALIBRATOR DILUENT II
WASH BUFFER (20X/96 wells, 30X/192 wells)
SUBSTRATE A
SUBSTRATE B
STOP SOLUTIONStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Interleukin-17A, Mouse, BioAssay ELISA Kit (IL-17A)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Interleukin-17A, Mouse, BioAssay ELISA Kit (IL-17A)产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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