- 中文名称
Interleukin-13, Human, BioAssay ELISA Kit (IL-13)
- 英文名字
- Interleukin-13, Human, BioAssay ELISA Kit (IL-13)
- 供应商
- USBiological
- 产品货号
- 216007
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- IL-13 is a 10kD unglycosylated protein, secreted mainly by activated T helper 2 cells. IL-13 binds to IL-4ra in addition to IL-13Ra1 and IL-13Ra2. And the function of IL-13 partially overlaps with IL-4. Both IL-4 and IL-13 link to signal transducer and activator of transcription 6 (STAT6) through the receptor activation. IL-13 can induce B-cell proliferation and IgE classs switching. However, IL-13 is less competent in this function comparing with IL-4. Unlike IL-4, IL-13 does not play an important role in hematopoietic cell shift, such as naïve T helper 0 differentiation to T helper 2. IL-I3 signaling is significant in inducing physiological changes to non-immuno cells in parasitized organs. For example, IL-13 induces enhanced gut contractions and glycoprotein hypersecretion which lead to the detachment of helminthes from gut wall. Eggs produced by Schistosoma mansoni can be limited inside granulomas formed through IL-13 induced cell changes. To organisms resided inside cells, IL-13 seemed to inhibit the clearance from host cell through antagonizing Th1 responses.IL-13 is also associated with many features of the allergic airway diseases, including mucus hypersecretion, goblet metaplasia, chemokine induction and recruitment of effector cells. All of them contribute to airway obstruction. Polymorphisms in IL-13 gene have been found to link to increased eosinophil count, serum total IgE and high risk of asthma. Intended Use:This Human Interleukin 13 ELISA Kit is to be used for the in vitro quantitative determination of human interleukin 13 concentrations in cell culture supernatant. Sensitivity:<2pg/mlRange:15.6-1000pg/mlTest Principle:This assay applies the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific to IL-13 has been pre-coated onto a microplate. Standards and samples are then added to the appropriate plate wells with a biotin-conjugated antibody preparation specific for IL-13 and incubated. IL-13 if present, will bind and become immobilized by the antibody pre-coated on the wells and then be "sandwiched" by biotin conjugate. Avidin is a tetramer containing four identical subunits that each has a high affinity-binding site for biotin. After washing away any unbound substances, avidin-Horseradish Peroxidase (HRP) will be added to each well and incubated. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and only those wells that contain IL-13, biotin conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The color development is stopped and the intensity of the color is measured.In order to measure the concentration of IL-13 in the samples, this package of reagent includes two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant testing.) According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D) versus IL-13 concentration. The concentration of IL-13 in the samples is then determined by comparing the O.D. of the samples to the standard curve.Kit Components:L-13 MICROTITER PLATE
BIOTIN CONJUGATE
AVIDIN CONJUGATE
IL-13 STANDARD
CALIBRATOR DILUENT I
CALIBRATOR DILUENT II
WASH BUFFER (20X/96 wells, 30X/192 wells)
SUBSTRATE A
SUBSTRATE B
STOP SOLUTIONStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Interleukin-13, Human, BioAssay ELISA Kit (IL-13)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Interleukin-13, Human, BioAssay ELISA Kit (IL-13)产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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