- 中文名称
Interferon-y, Mouse, BioAssay ELISA Kit (IFN-y)
- 英文名字
- Interferon-y, Mouse, BioAssay ELISA Kit (IFN-y)
- 供应商
- USBiological
- 产品货号
- 216003
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- IFN-ã is a multifunctional cytokine, possessing important immune-modulation, anti-viral and anti-tumor properties. It is produced by natural killer cells and natural killer T cells, plays an important part in innate immunity. CD4+ T helper 1 cells, CD8 cytotoxic T lymphocyte (CTL) and effecter T cells also produce IFN-y when acquired immunity develops. IFN-ã stimulates its own expression and up-regulates other genes through the Jak-Stat signaling pathway. IFN-y released after viral infection stimulates the production of large amount of protein kinase R, which phosphorylates transcription initiation factor elF in response to viral infection. As a results, enzymes critical to mRNA replication is reduced and mRNA replication inhibited. Experiment with murine cytomegalovirus infected mice (1) showed that natural killer cells are the main source of IFN-y. In natural killer cell depleted mice, viral hepatitis and viral replication levels were higher than control group. The experiment also demonstrated that inhibition of viral replication is mediated by IFN-a, since inhibition is independent of cytolytic activity. In the same study, administration of IL-12 to the MCMV infected mice was found to induce the production of IFN-ã by natural killer cells. Previously (3), interleukin IL-12 has been identified, by Kobayashi et al, as natural killer cell stimulatory factor released from Epstein-Barr-virus infected B cells.IFN-y influences the emergence of cellular immunity through stimulating the maturation of CD4+ T helper 1 cells (Th1) and cytotoxic CD8+ cells (2). IFN-y suppresses T helper 2 differentiation and humoral immunity, and was also found to regulate mouse subclass switching (5). IFN-y is a potent macrophage activator. It binds to macrophage in conjugation with CD40, causing the macrophage to produce elevated amounts of MHCmolecules. It also stimulates the production of antigen-processing associated transporters and enzymes. The accumulation of macrophages and T helper 1 cells around M. tuberculosis infected cells is believed to the mechanism for granuloma formation in TB patients. IFN-y was found to limit infection (4) in chronic granulomatous diseases (CGD). A form of this cytokine has been approved by FDA for treatment of CGD.Inhibition to cancer growth by IFN-y is well documented. Recombinant IFN-y has been used for treatment of various cancer types in combination with chemotherapy. Recent experiment with mouse skin cancer model showed that IFN-y plasmidDNAhad inhibitory effects in skin carcinogenesis (6), indicating IFN-y DNAcould be useful for cancer gene therapy.This mouse IFN-y ELISA is a 4.5 hour solid phase immunoassay readily applicable to measure IFN-y levels in mouse serum, cell culture supernatant, and other biological fluids in the range of 0 to 3200pg/ml. It is expected to be useful for investigations into the relationship between IFN-y and various diseases.Intended Use:This Mouse IFN-y ELISA kit is to be used for the in vitro quantitative determination of mouse interferon-y concentrations in mouse serum and cell culture supernatant. Sensitivity:<7.5pg/mlRange:50-3200pg/mlTest Principle:This mouse IFN-y enzyme-linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre- coated with a monoclonal specific for mouse IFN-u. Standards or samples are then added to the appropriate microtiter plate wells and incubated. In order to quantitatively determine the amount of mouse IFN-u present in the sample, a biotin-conjugated antibody specific for mouse IFN-y is added to each well to "sandwich" the IFN-u immobilized during the first incubation. The microtiter plate then undergoes a second incubation and the wells are thoroughly washed to remove all unbound Biotin-conjugated antibodies. Avidin can bind to biotin with high affinity and amplify the biotin signal. A preparation of avidin conjugated HRP is added to each well and incubated. The avidin HRP conjugate will bind to wells containing biotin. After washing the wells, the unbounded avidin-HRP conjugate is removed. A TMB (3,3'5, 5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IFN-y and biotin-conjugated antibody, avidin conjugated HRP will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.In order to measure the concentration of mouse IFN-y in the samples this kit contains two calibration diluents. (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant). According to the testing system, the provided standard is 2 x fold serial diluted with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D.) versus mouse IFN-y concentration (pg/ml). The concentration of mouse IFN-y in the samples is then determined by comparing the O.D. of the samples to the standard curve.Kit Components:MOUSE IFn-y MICROTITER PLATE
MOUSE IFn-y BIOTIN CONJUGATE
AVIDIN HRP CONJUGATE
MOUSE IFn-y STANDARD
CALIBRATOR DILUENT I
CALIBRATOR DILUENT II
WASH BUFFER (20X/96 wells, 30X/192 wells)
SUBSTRATE A
SUBSTRATE B
STOP SOLUTIONStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Interferon-y, Mouse, BioAssay ELISA Kit (IFN-y)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Interferon-y, Mouse, BioAssay ELISA Kit (IFN-y)产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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