- 中文名称
Granulocyte Macrophage Colony Stimulating Factor, Mouse, BioAssay ELISA Kit (GM-CSF)
- 英文名字
- Granulocyte Macrophage Colony Stimulating Factor, Mouse, BioAssay ELISA Kit (GM-CSF)
- 供应商
- USBiological
- 产品货号
- 215993
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) is a member of the hematopoietic cytokine family, which includes interleukin-3 (IL-3) and interleukin-5 (IL-5). The non-glycosylated recombinant mouse GM-CSF has a calculated molecular weight of 14kD. Because of the formation of intramolelular disulfide bonds and glycosylation, GM-CSF migrates at different sizes on SDSPAGE gel. Human GM-CSF shows 56-60% amino acid homology to murine GM-CSF but does not exhibit cross-species biological activity or receptor binding. GM-CSF is a potent stimulator to the marrow granulocyte/monocyte progenitor cells and promotes the function of mature granulocytes and monocytes. GM-CSF is synthesized upon stimulation by inflammatory agent or antigen. GM-CSF can be produced by and act upon a variety cell types, including T-lymphocytes, monocytes/macrophages, B-lymphocytes, endothelial cells, fibroblasts, stromal cells, mesothelial cells, keratinocytes, osteoblasts, uterine epithelial cells, synoviocytes, mast cells and various solid tumors. Mouse GM-CSF receptor is comprised of a unique alpha subunit and a common beta subunit which is shared by IL-3 and IL-5 receptor. GM-CSF receptor is abundant on progenitor and mature granulocytes and monocytes. The low level expression of GM-CSF receptor on non-hematopoietic cells may explain the phenomenon that higher GM-CSF concentration is required for producing biological effects on these cells. GM-CSF stimulates macrophage production of TNF, M-CSF, G-CSF, and IL-1, and promotes the killing of infectious agents by granulocytes and macrophages. Increased GM-CSF levels were found to be associated with various inflammatory and pathological conditions including pneumonia, asthma, psoriasis, pulmonary fibrosis, rheumatoid arthritis, systemic lupus erythematosus, myelodysplastic syndrome (MDS), thrombocytopenia, lung cancer, acute mylogenous leukemia, and tumor related thrombocytosis, GM-CSF showed therapeutic value in accelerating neutrophil recovery at myelo-suppression conditions, such as bone marrow transplantation, chemotherapy, and infectious disease.This ELISA kit provides a tool for studying GM-CSF expression and regulation in animal model.Intended Use:This Mouse GM-CSF ELISA Kit is to be used for the in vitro quantitative determination of mouse granulocyte macrophage colony stimulating factor (GM-CSF) concentrations in serum, plasma, cell culture supernatant, and other biological fluids.Sensitivity:<17pg/mlRange:50-3200pg/mlTest Principle:This mouse GM-CSF enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for mouse GM-CSF. Standards or samples are then added to the appropriate microtiter plate wells and incubated. Mouse GM-CSF, if present, will bind and become immobilized by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed to remove unbound mouse GM- CSF and other components of the sample. In order to quantitatively determine the amount of mouse GM-CSF present in the sample, a standardized preparation of horseradish peroxidase HRP-conjugated monoclonal antibody specific for mouse GM-CSF is added to each well to “sandwich” the mouse GM-CSF immobilized during the first incubation. The microtiter plate then undergoes a second incubation. The wells are thoroughly washed to remove all unbound HRP-conjugated antibodies and a TMB (3,3’5,5’ tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain mouse GM-CSF and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 ± 2nm.In order to measure the concentration of mouse GM-CSF in the samples, this kit includes two diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant testing). According to the testing system, the provided standard is diluted (2-fold dilution series) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D.) versus mouse GM-CSF concentration (pg/ml). The concentration of mouse GM-CSF in the samples is then determined by comparing the O.D. of the samples to the standard curve.This mouse GM-CSF ELISA is a 3.5-hour solid phase immunoassay readily applicable to measure mouse GM-CSF in serum, plasma, cell culture supernatant, and other biological fluids in the range of 0 to 3200pg/ml. It showed no cross reactivity with other cytokines tested.Kit Components:MOUSE GM-CSF MICROTITER PLATEBIOTIN CONJUGATEAVIDIN CONJUGATEMOUSE GM-CSF STANDARD CALIBRATOR DILUENT ICALIBRATOR DILUENT IIWASH BUFFER (20X/96 wells, 30X/192 wells) SUBSTRATE A SUBSTRATE B STOP SOLUTIONStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Granulocyte Macrophage Colony Stimulating Factor, Mouse, BioAssay ELISA Kit (GM-CSF)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Granulocyte Macrophage Colony Stimulating Factor, Mouse, BioAssay ELISA Kit (GM-CSF)产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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