Pertussis Toxin, Mutant-Pertussis Toxin, Mutant代理/参数/厂家-生化试剂-艾美捷科技有限公司

PRODUCT CENTER

生化试剂

产品中心

当前位置：首页 > 产品中心 > 分子生化产品 > 生化试剂

背景资料: USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！

产品描述: The Pertussis Toxin Mutant contains a modified sequence encoding the enzyme subunit. Virulence of this pertussis mutant is greatly reduced relative to that found with the wild type. The pertussis mutant protein is isolated from the TY-178 strain of Bordetella bronchiseptica which contains a genetically modified sequence encoding the S1 sub unit. Site-directed mutagenesis was used to replace amino acid residues arginine-9 with lysine (R9K) and glutamic acid-129 with alanine (E129A). Genetically inactivated toxin has reduced activity relative to the native toxin and may be used as an antigen or as a carrier to promote an immune response.Source: Pertussis Toxin, Mutant, strain TY-178 from Bordetella bronchisepticaBiological Activity: The lowest concentration of toxin at which a positive response (clustered growth pattern) was obtained was 3.3ng/ml. The adenylate cyclase activity of this lot, in the presence of 1umolar calmodulin, is 1picomole cAMP/min/ug Pertussis Toxin Mutant when assayed by the method of Wolff et al. Endotoxin:~20EU/mg (Kinetic Chromagenic LAL assay)Storage and Stability:Lyophilized powder may be stored at 4°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at 4°C. Reconstituted product is stable for 6 months at 4°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

产品形式: 该产品为冻干粉 in 0.01M sodium phosphate, pH 7.0, 0.05M sodium chloride，缓冲液为：100ul sterile ddH2O。 Handle Gently。Do not vortex。

其他: Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。