中文名称

Adenylate Cyclase Antigen, Native, B. pertussis

英文名字

Adenylate Cyclase Antigen, Native, B. pertussis

供应商

USBiological

产品货号

215895

产品报价

￥1/50ug

产品说明书

点击查看

购买方式

银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868

产品新闻

背景资料

USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！

产品描述

Adenylate cyclase toxin (ACT) is an important virulence factor secreted by Bordetella pertussis. When a lung infection is underway, ACT interacts with tracheal epithelial cells, inserting itself into cytoplasmic membranes, aiding the adhesion of bacteria to the airway lining. Host phagocytes responding to the site of infection are disabled by cAMP generated by ACT. Encoded by the Bordetella pertussis cyaA gene, adenylate cyclase toxin is single 1706aa polypeptide, with an apparent molecular weight of 220kD. The N-terminal of this protein contains an adenylate cyclase domain which binds to host cell calmodulin and catalyzes unregulated conversion of cellular ATP to cAMP. The C-terminal, receptor binding domain, contains numerous repeat in toxin (RTX) motifs and is responsible for binding to the cellular receptor on the surface of immune cells, translocation to the cell cytosol and creating cation-selective pores in the host cell membrane.Adenylate cyclase toxin both suppresses and modulates the host immune system contributing to pathogenesis of B. pertussis. This toxin is unusual in its ability to cross mammalian cell membranes and locate within the cytosol. As a cell biology tool, adenylate cyclase has been used to deliver immunogenic epitopes, targeting antigen presenting cells. This protein is the native cell surface antigen but does not exhibit the level of enzyme activity shown by ACT produced in E. coli. In Western Blot assays, this product reacts to adenylate cyclase toxin antibodies.  Source: Adenylate Cyclase Antigen, extracted from native Bordetella pertussis cells. Activity:The enzymatic activity of this preparation is ~160umoles of cAMP/min/mg protein as determined by a modification of the method of Hewlett et. at. Storage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

产品形式

该产品为冻干粉 in 20mM HEPES, pH 7.5, 8M urea，缓冲液为：250ul sterile ddH2O。Handle Gently。Do not vortex。

保存建议

接收到Adenylate Cyclase Antigen, Native, B. pertussis 产品后，置于-20°C温度下保存。

其他

Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。

注意

该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。

分享到：