中文名称

Pertussis Toxin (Islet Activating Protein, B. pertussis)

英文名字

Pertussis Toxin (Islet Activating Protein, B. pertussis)

供应商

USBiological

产品货号

209138

产品报价

￥1/50ug

产品说明书

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购买方式

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产品新闻

背景资料

USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！

产品描述

Pertussis toxin (PT) is a protein-based AB5-type exotoxin produced by the bacterium Bordetella pertussis, which causes whooping cough. PT is involved in the colonization of the respiratory tract and the establishment of infection. PT may have a therapeutic role in treating a number of common human ailments, including hypertension, viral inhibition, and autoimmune inhibition.A large group of bacterial exotoxins are referred to as "A/B toxins", in essence because they are formed from two subunits. The "A" subunit possesses enzyme activity, and is transferred to the host cell following a conformational change in the membrane-bound transport "B" subunit. Pertussis toxin is an exotoxin with six subunits (named S1 through S5-each complex contains two copies of S4). The subunits are arranged in A-B structure: the A component is enzymatically active and is formed from the S1 subunit, while the B component is the receptor-binding portion and is made up of subunits S2-S5. The subunits are encoded by ptx genes encoded on a large PT operon that also includes additional genes that encode Ptl proteins. Together, these proteins form the PT secretion complex.B. pertussis expresses two forms of a rough-type lipooligosaccharide (LOS), which have been referred to as LOS A and LOS B. The two forms of LOS can be resolved as two distinct molecular weight species in silver stained sodium dodecyl sulfate-polyacrylamide gels of protease-treated B. pertussis cell lysates. The difference between the two forms of LOS has been shown to be due to three additional N-acetyl amino sugars in the core oligosaccharide moiety of LOS A that are not present in LOS B. The structure and biological activity of B. pertussis LOS are similar to those of LOSs of other gram negative bacteria that contain lipid A and a core oligosaccharide moiety containing 2-keto-3-deoxyoctonate, exhibiting lethal toxicity in mice, pyrogenicity in rabbits, and mitogenicity in cell cultures. Toxoid refers to the inactivated version of the active toxin. It is typically produced by formaldehyde inactivation of the active protein.Source:Pertussis toxin, from B. pertussisBiological Activity:The lowest concentration of toxin at which a positive response (clustered growth pattern) was obtained was 0.33ng/ml in CHO cells. Specific Activity:The adenylate cyclase activity of this lot is 4.5 pmole/min/ug in the presence of 1uM calmodulin.Applications: Suitable for use in ELISA, Western Blot, Antibody Titration or as control protein for Adjuvant. Other applications not tested.Recommended Dilution:Optimal dilutions to be determined by the researcher.Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

产品形式

该产品为液体形式，缓冲液为：0.05M Tris, 0.01M glycine, 0.5M NaCI, pH 7.5, 50% glycerol。

保存建议

接收到Pertussis Toxin (Islet Activating Protein, B. pertussis)产品后，置于-20°C温度下保存。

其他

Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。

注意

该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。

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