中文名称

Horseradish Peroxidase (HRP)

英文名字

Horseradish Peroxidase (HRP)

供应商

USBiological

产品货号

209011

产品报价

￥1/1mg

产品说明书

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购买方式

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产品新闻

背景资料

USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！

产品描述

Horseradish peroxidase (HRP) is isolated from horseradish roots (Amoracia rusticana) and belongs to the ferroprotoporphyrin group of peroxidases. HRP is a single chain polypeptide containing four disulfide bridges. It is a glycoprotein containing 18% carbohydrate. The carbohydrate compositionconsistsof galactose, arabinose, xylose, fucose, mannose, mannosamine, and galactosamine, depending upon the specific isozyme.2 Its molecular weight (~44kD) includes the polypeptide chain (33,890 Daltons), hemin plus Ca2+ (approx. 700 Daltons), and carbo-hydrate (9400 Daltons).3 At least seven isozymes of HRP exist.2 The isoelectric point for horseradish Peroxidase isozymes ranges from 3-9. HRP readily combines with hydrogen peroxide (H2O2) and the resultant [HRP-H2O2] complex can oxidize a wide variety of chromogenic hydrogen donors. It can also utilize chemiluminescent substrates such as luminol and isoluminol and fluorogenic substrates such as tyramine, homovanillic acid, 4-hydroxyphenyl acetic acid. The following compounds are inhibitors of horseradish peroxidase: sodium azide, cyanide, L-cystine, dichromate, ethylenethiourea, hydroxylamine, sulfide,vanadate, paminobenzoic acid, Cd+2, Co+2, Cu+2, Fe+3, Mn+2, Ni+2, Pb+2. 4 The pH optimum of HRP is in the range of 6.0 to 6.5; activity at 7.5 is 84% of the maximum. The enzyme is most stable in the pH range of 5.0 to 9.0.5 Horseradish peroxidase is widely used as a label for immunoglobulins in many different immunochemistry leads to lower non-specific binding.6 Protocols describing the glutaraldehyde and periodate conjugation methodologies can be reviewed in Harlow, E. et al.Source:HRP, from horseradish roots (Amoracia rusticana)RZ (Reinheitszahl): Absorbance ratio of A403/A275. RZ=3.0.Purpurogallin Activity (units per mg solid):~250-330Purpurogallin Unit Definition:One unit will form 1.0mg purpurogallin from pyrogallol in 20 seconds at pH 6.0 at 20 °C. This unit is equivalent to approx. 18uM units/minute at 25 °C.ABTS Unit Definition: One unit will oxidize 1umole of 2, 2’-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)/minute at 25°C, pH 5.0.ABTS Activity (units per mg solid): ~1000Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

产品形式

该产品为冻干粉形式。 The choice of solvent will depend on the intended application。The 粉末ed enzymes are soluble in H2O or 0.1M phosphate buffer, pH 6 (10mg/ml)。

保存建议

接收到Horseradish Peroxidase (HRP)产品后，置于0°C温度下保存。

其他

Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。

注意

该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。

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