FIH, Factor inhibiting HIF1 (hypoxia-inducible factor), is an asparaginyl hydroxylase. FIH in conjunction with VHL represses HIF-1 transcriptional activity by disrupting the interaction of HIF-1 with the transcriptional coactivators CBP/p300, and by recruiting histone deacetylases. FIH activity is inhibited during hypoxia. Recent studies show that low nuclear expression of FIH is a strong independent prognostic factor for a poor overall survival in clear cell renal cell cancinoma.
应用类型
Immunohistochemistry,Western Blot,
免疫原
FIH Antibody was produced in mice by repeated immunizations with full length human FIH expressed in E.coli BL21 (DE3) cells.
来源宿主
Mouse
反应性
H. sapiens (Human)
保存建议
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
