中文名称

Glucose Oxidase (GOx, Glucose oxyhydrase, Beta-D-glucose, Oxygen 1-oxidoreductaase)

英文名字

Glucose Oxidase (GOx, Glucose oxyhydrase, Beta-D-glucose, Oxygen 1-oxidoreductaase)

供应商

USBiological

产品货号

208915

产品报价

￥1/250mg

产品说明书

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购买方式

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产品新闻

背景资料

USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！

产品描述

The glucose oxidase enzyme (GOx) binds to beta-D-glucose (an isomer of the six-carbon sugar glucose) and aids in breaking the sugar down into its metabolites. GOx is a dimeric protein (605aa; ~160Kd with one tightly bound FAD per monomer, K=1x10-10). Gox is glycosylated with a carbohydrate content of 16% (w/w). The carbohydrate moiety is designated as high mannose type with 80% (w/w) of the carbohydrate being mannose. The mannose is N and O glycosidically linked to Asn, Thr and Ser. The protein is readily soluble in 0.1M potassium phosphate pH 7.0 giving a clear, yellow solution. The molar extinction coefficient of a 1% (w/v) solution at 280nm is 13.8. The enzyme shows a very high degree of specificity for b-D-glucose although 2-deoxy-D- glucose, D-mannose and D-fructose are also oxidised, albeit at a much reduced rate. The native protein is acidic having an isoelectric point (pI) of 4.2. GOx catalyzes the oxidation of beta-D-glucose into D- glucono-1,5-lactone, which then hydrolyzes to gluconic acid. In order to work as a catalyst, GOx requires a cofactor, flavin adenine dinucleotide (FAD). FAD is a common component in biological oxidation-reduction (redox reactions). Redox reactions involve a gain or loss of electrons from a molecule. In the GOx-catalyzed redox reaction, FAD works as the initial electron acceptor and is reduced to FADH2. Then FADH2 is oxidized by the final electron acceptor, molecular oxygen (O2), which can do so because it has a higher reduction potential. O2 is then reduced to hydrogen peroxide (H2O2). The glucose oxidase enzyme is commonly used in biosensors to detect levels of glucose by keeping track of the number of electrons passed through the enzyme by connecting it to an electrode and measuring the resulting charge. When produced commercially for this application, it is often extracted from Aspergillus niger. Glucose oxidase is widely used for the determination of glucose in body fluids and in removing residual glucose and oxygen from beverages and foodstuffs. Furthermore, Glucose oxidase-producing moulds such as Aspergillus and Penicillum Species are used for the biological production of gluconic acid.Source:Glucose Oxidase from Aspergillus nigerMolecular Weight:~160kD Enzyme Activity: ~300 units/mg protein or ~200 units/mg solid material, average 225-275.Inhibitors: Ag+, Hg2+, Cu2+. FAD binding is inhibited by several nucleotidesUnit Definition: 1 unit producing oxidation of 1 umole of glucose per minute at 25°C and pH 7.0. 1 unit is 0.6 units in the system below, but without oxygenation. 0.6 units using 4-AA-phenol coupled with HRP at pH 5.6 and 30°C. 0.63 titrimetric units at pH 5.1 and 35°C.Solubility: Dissolve readily at 5mg/ml in 0.1M potassium phosphate, pH 7.0. Contaminants:Alpha-amylase, Saccharase, Maltase, <0.2% Catalase <10 U/mg GO/Cat: >25Storage and Stability:Store lyophilized powder dessicated at 20°C. Stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. This product is sensitive to light.

产品形式

该产品为冻干粉形式。 No preservative added。

保存建议

接收到Glucose Oxidase (GOx, Glucose oxyhydrase, Beta-D-glucose, Oxygen 1-oxidoreductaase)产品后，置于-20°C温度下保存。

其他

Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。

注意

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