中文名称

CRM197, Diphtheria Toxin Mutant

英文名字

CRM197, Diphtheria Toxin Mutant

供应商

USBiological

产品货号

208815

产品报价

￥1/100ug

产品说明书

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购买方式

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产品新闻

背景资料

USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！

产品描述

Diphteria is a localized infection of mucous membranes or skin caused by toxigenic strains of CORYNEBACTERIUM DIPHTHERIAE. It is characterized by the presence of a pseudomembrane at the site of infection. DIPHTHERIA TOXIN, produced by C. diphtheriae, can cause myocarditis, polyneuritis, and other systemic toxic effects. Diphtheria toxin is an exotoxin secreted by Corynebacterium diphtheriae, the pathogen bacterium that causes diphtheria. Diphtheria toxin is a single polypeptide chain of 535aa (~63kD) consisting of two subunits linked by disulfide bridges. An ADP-ribosylating polypeptide produced by CORYNEBACTERIUM DIPHTHERIAE that causes the signs and symptoms of DIPHTHERIA. It can be broken into two unequal domains: the smaller (24 kda), catalytic A domain is the lethal moiety and contains MONO(ADP- RIBOSE) TRANSFERASES which transfers ADP RIBOSE to PEPTIDE ELONGATION FACTOR 2 thereby inhibiting protein synthesis; and the larger B domain (39kD) that is needed for entry into cells. Binding to the cell surface of the less stable of these two subunits allows the more stable part of the protein to penetrate the host cell. It catalyzes the ADP-ribosylation of eukaryotic elongation factor-2 (eEF2), inactivating this protein. It does so by ADP-ribosylating the unusual aminoacid diphthamide. In this way, it acts as a RNA translational inhibitor. The exotoxin A of Pseudomonas aeruginosa uses a similar mechanism of action. Diphtheria toxin is extraordinarily potent.[1] The lethal dose for humans is about 0.1ug of toxin per kg of bodyweight. A massive release of toxin into the body will likely cause lethal necrosis of the heart and liver. Diphtheria Toxoid is formaldehyde-inactivated toxin of Corynebacterium diphtheriae. It is generally used in mixtures with TETANUS TOXOID and PERTUSSIS VACCINE; (DTP); or with tetanus toxoid alone (DT for pediatric use and Td, which contains 5- to 10-fold less diphtheria toxoid, for other use). Diphtheria toxoid is used for the prevention of diphtheria; DIPHTHERIA ANTITOXIN is for treatment. Mutant forms of diphtheria toxin (DT), cross-reactive material 197 (CRM197) is a non-toxic DT mutant containing a lesion in the A chain blocking ADP-ribosylation. CRM results from a single base change in the structural gene resulting in the substitution of glutamic acid for glycine. While CRM shows no enzymatic activity, it is immunologically indistinguishable from diphtheria toxin. In its applications, CRM 197 is similar to diphtheria toxoid. CRM has the advantage of being a well defined protein in contrast to formaldehyde treated toxin (toxoid) which is non-specifically cross linked and subject to rearrangement. CRM functions as a carrier for polysaccharides and haptens making them immunogenic.Source:CRM197, from mutant Diphtheria Toxin (DT)Applications: Suitable for use in ELISA, Western Blot, Antibody Titration or as Control Protein for Adjuvant. Other applications not tested.Recommended Dilution:Optimal dilutions to be determined by the researcher.Storage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

产品形式

该产品为冻干粉 in 10mM phosphate buffer, pH 7.5, 1mM EDTA, 5% lactose，缓冲液为：sterile ddH2O。

保存建议

接收到CRM197, Diphtheria Toxin Mutant 产品后，置于-20°C温度下保存。

其他

Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。

注意

该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。

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