中文名称

Alkaline Phosphatase, Calf Intestine, (3000-6000u/mg) (AP, Free of Endonuclease, Exonuclease and RNase Activities)

英文名字

Alkaline Phosphatase, Calf Intestine, (3000-6000u/mg) (AP, Free of Endonuclease, Exonuclease and RNase Activities)

供应商

USBiological

产品货号

208733

产品报价

￥1/1mg   ￥1/10mg

产品说明书

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购买方式

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产品新闻

背景资料

USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！

产品描述

Alkaline phosphatases (APs) are highly ubiquitous enzymes, present in all species from bacteria to man. In humans, APs are encoded by a multi-gene family composed of four loci; i.e., tissue- nonspecific AP, also called bone/liver/kidney AP, (Weiss et al, J. Biol. Chem., 264, 12002-12010, 1988), intestinal (Henthorn et. al., J.Biol. Chem., 263, 12020-12027, 1988). The sequence and complexity of the AP genes from other vertebrates and lower species are now being elucidated. The biological function of AP isozymes is still unknown. In vitro, the enzymes behave as phosphotransferases at neutral pH. The use of phosphate acceptor molecules (diethanolamine, tris, 2-amino-2-methyl-1- propanol) in the buffered substrate solutions increases the reaction rates and, thus, the sensitivity of assays based on AP determinations. ALP is commonly used as a label in immunoassays such as ELISA, and in blotting and histochemistry. Once conjugated to antibodies, antigens, or streptavidin, its low backgrounds and linear reaction rate enables increased sensitivity over extended incubation times. It can be used with a variety of substrates producing precipitated or soluble chromogens, or with chemiluminescent substrates for enhanced sensitivity.Source:Alkaline phosphatase, from Calf IntestineActivity:~3000-6000U/mg proteinUnit Definition:Glycine Units:The amount of enzyme causing the hydrolysis of one micromole of p-Nitrophenyl phosphate/minute at pH 9.6 and 25°C (glycine buffer).DEA Units:The amount of enzyme causing the hydrolysis of one micromole of p-Nitrophenyl phosphate/minute at pH 9.8 and 37°C (diethanolamine buffer).Unit Conversion:One Glycine unit as described above is equivalent to ~three DEA units at pH 9.8 and 37°C.Storage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

产品形式

该产品为冻干粉形式。 No preservative added，缓冲液为：sterile ddH2O。

保存建议

接收到Alkaline Phosphatase, Calf Intestine, (3000-6000u/mg) (AP, Free of Endonuclease, Exonuclease and RNase Activities)产品后，置于-20°C温度下保存。

其他

Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。

注意

该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。

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