- 中文名称
IL-1alpha, Mouse, BioAssay ELISA Kit (Interleukin-1alpha IL-1F1)
- 英文名字
- IL-1alpha, Mouse, BioAssay ELISA Kit (Interleukin-1alpha IL-1F1)
- 供应商
- USBiological
- 产品货号
- 207234
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- The most prominent members of the interleukin-1 (IL-1) superfamily are IL-1alpha and IL-1beta. They lack a signal peptide and are secreted by an unconventional, endoplasmic reticulum-Golgi-independent mechanism. IL-1alpha was reported to be more widely and constitutively expressed and has intracellular functions, but also acts locally in a membrane-bound form by activating IL-1R1. Additionally, passive release of IL-1alpha upon cell death can trigger a sterile inflammatory response to dying cells. The cleavage of IL-1alpha is not mediated by caspase-1 and is not re- quired for binding to IL-1R1. Recently it has been observed that all activators of the inflammasome NLRP3/NALP3 induce the simultaneous secretion of IL-1alpha and IL-1beta. Although most activators fully rely on the inflammasome for IL-1alpha secretion, some induce the processing and secretion of IL-1alpha in an inflammasome-independent mannerSample Type:Cell culture supernatants, serum and plasmaIntended Use:The mouse IL-1 alpha BioAssay ELISA Kit is to be used for the in vitro quantitative determination of mouse IL-1alpha in cell culture supernatants, serum and plasma. Sensitivity:9pg/mlRange:15.6pg/ml-1ng/mlSpecificity:Recognizes natural and recombinant mouse IL-1alpha (proform and p17 fragment). Does not detect human IL-1alpha or mouse IL-1beta.Test Principle:This BioAssay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of mouse IL-1alpha in cell culture supernatants, serum and plasma. A monoclonal antibody specific for IL-1alpha has been precoated onto the 96-well microtiter plate. Standards (STD) and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, IL-1alpha is recognized by the addition of a biotinylated monoclonal antibody specific for IL-1alpha (DET). After removal of excess biotinylated antibody, streptavidine-peroxidase (STREP-HRP) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3’,5,5’-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450nm after acidification and is directly proportional to the concentration of IL-1alpha in the samples.Kit Components:Mouse IL-1alpha Standard, lyophilized, 1x1ugDetection Antibody, 1x20ulHRP Labeled Streptavidin, lyophilized, 1x2ugWash Buffer 10X, 2x30mlELISA Buffer 10X, 2x30mlTMB Substrate Solution, 1x12mlStop Solution, 1x12mlPlate coated with mIL-1alpha antibody, 6x16 well stripsPlate covers, 2x plastic filmSilica gel, 2x minibagsStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对IL-1alpha, Mouse, BioAssay ELISA Kit (Interleukin-1alpha IL-1F1)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到IL-1alpha, Mouse, BioAssay ELISA Kit (Interleukin-1alpha IL-1F1)产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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