- 中文名称
Vascular Cell Adhesion Molecule 1 (VCAM-1) BioAssay ELISA Kit (Rabbit) discontinued
- 英文名字
- Vascular Cell Adhesion Molecule 1 (VCAM-1) BioAssay ELISA Kit (Rabbit) discontinued
- 供应商
- USBiological
- 产品货号
- 206379
- 产品报价
- ￥1/48Tests ￥1/96Tests
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- The BioAssay ELISA Kit is a 1.5 hour solid-phase Competitive ELISA designed for the quantitative determination of Vascular Cell Adhesion Molecule 1 (VCAM-1) (Rabbit) in serum, plasma, cell culture supernatants, cell lysates, body fluid and tissue homogenates.Detection Range:5.0-100ng/mlSensitivity:0.1ng/mlPrecision:Intra-Assay CV: <10%Inter-Assay CV: <10%Specificity:This assay has high sensitivity and excellent specificity for detection of Vascular Cell Adhesion Molecule 1 (VCAM-1) (Rabbit). No significant crossreactivity or interference with analogs was observed. Note: Crossreactivity detection between the Target Protein and all analogs has not been determined and crossreaction may exist in some cases.Assay Principle:Vascular Cell Adhesion Molecule 1 (VCAM-1) BioAssay ELISA Kit (Rabbit) utilizes the quantitative Competitive Enzyme Immunoassay technique, utilizing a monoclonal antibody and an conjugate to Vascular Cell Adhesion Molecule 1 (VCAM-1) (Rabbit). The assay sample and buffer are incubated together with the HRP conjugate in a pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Target Protein concentration since VCAM-1 from samples and VCAM-1-HRP conjugate compete for the anti-VCAM-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by VCAM-1 from the sample, fewer sites are left to bind the VCAM-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The target protein concentration in each sample is interpolated from this standard curve.Kit Components:206379A: Microtiter Strips, 1x48 or 1x96 wells206379B: Enzyme Conjugate, 1x3ml206379C: Standard, 0pg/ml, 1x1 vial206379D: Standard, 5.0ng/ml, 1x1 vial206379E: Standard, 10ng/ml, 1x1 vial206379F: Standard, 25ng/ml, 1x1 vial206379G: Standard, 50ng/ml, 1x1 vial206379H: Standard, 100ng/ml, 1x1 vial206379J: Substrate A, 1x6ml206379K: Substrate B, 1x6ml206379L: Stop Solution, 1x6ml206379M: Wash Solution, 100X, 1x10ml206379N: Balance Solution, 1x3mlStorage and Stability:Store components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- 产品特点
- 针对Vascular Cell Adhesion Molecule 1 (VCAM-1) BioAssay ELISA Kit (Rabbit) discontinued该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Vascular Cell Adhesion Molecule 1 (VCAM-1) BioAssay ELISA Kit (Rabbit) discontinued产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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