Anti-streptavidin antibody detects Streptavidin, a 60 kDa protein purified from the bacterium Streptomyces avidinii. Streptavidin is a homo-tetrameric protein having a very high affinity for biotin (vitamin B7). Streptavidin bound biotin has a dissociation constant (Kd) on the order of about 10^−14 mol/L. Streptavidin and biotin are used extensively in molecular biology and bionanotechnology due to the resistance of streptavidin-biotin complex to organic solvents, denaturants (e.g. guanidinium chloride), detergents (e.g. SDS, Triton), proteolytic enzymes, and extremes of temperature and pH.
应用类型
ELISA,Western Blot,
免疫原
Streptavidin [Streptomyces avidinii]
来源宿主
Rabbit
反应性
Streptomyces avidinii
保存建议
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
