Pancreatic ribonuclease is an endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. It acts on single-stranded and double-stranded RNA. This ribonuclease can destabilize or unwind the DNA helix by complexing with single-stranded DNA; this complex arises by an extended multisite cation-anion interaction between the lysine and arginine residues of the enzyme and the phosphate groups of the nucleotides. It has endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates.
应用类型
ELISA,Immunoprecipitation,Western Blot,
免疫原
Ribonuclease A [Bovine pancreas]
来源宿主
Rabbit
反应性
Bovine (Bos Taurus)
保存建议
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
