- 中文名称
L-Lactate Dehydrogenase B Chain (LDHB) BioAssay ELISA Kit (Rat) discontinued
- 英文名字
- L-Lactate Dehydrogenase B Chain (LDHB) BioAssay ELISA Kit (Rat) discontinued
- 供应商
- USBiological
- 产品货号
- 195723
- 产品报价
- ¥1/48Tests ¥1/96Tests

- 产品说明书
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- 产品新闻

- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理,是行业中少有的致力于服务客户,帮助客户,且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应,专业的产品和解决方案支持,稳健快捷的交货周期,优质放心的售后服务。我们致力于为您提供有价值的产品和服务,在意您的成功!
- 产品描述
- The L-Lactate Dehydrogenase B Chain (LDHB) BioAssay ELISA Kit (Rat) is a 1.5 hour solid-phase Competitive Enzyme Immunoassay designed for the quantitative determination of L-Lactate Dehydrogenase B Chain (LDHB) (Rat) in serum, plasma, cell culture supernatants, body fluid and tissue homogenates.Detection Range:25-500mU/mlSensitivity:1.0mU/mlPrecision:Intra-Assay CV: <10%Inter-Assay CV: <10%Specificity:This assay has high sensitivity and excellent specificity for detection of L-Lactate Dehydrogenase B Chain (LDHB) (Rat). No significant crossreactivity or interference with analogs was observed. Note: Crossreactivity detection between the LDHB and all analogs has not been determined and crossreaction may exist in some cases.Kit Components:195723A: Microtiter Plate, 48 wells, stripwell195723B: Enzyme Conjugate, 1x3ml195723C: Standard A, 0mU/ml, 1x1 vial195723D: Standard B, 25mU/ml, 1x1 vial195723E: Standard C, 50mU/ml, 1x1 vial195723F: Standard D, 100mU/ml, 1x1 vial195723G: Standard E, 250mU/ml, 1x1 vial195723H: Standard F, 500mU/ml, 1x1 vial195723J: Substrate A, 1x6ml195723K: Substrate B, 1x6ml195723L: Stop Solution, 1x6ml195723M: Wash Solution (100X), 1x10ml195723N: Balance Solution, 1x3ml Storage and Stability:Store components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.Assay Principle:L-Lactate Dehydrogenase B Chain (LDHB) BioAssay ELISA Kit (Rat) utilizes the quantitative Competitive Enzyme Immunoassay technique, utilizing a monoclonal antibody and an conjugate to L-Lactate Dehydrogenase B Chain (LDHB) (Rat). The assay sample and buffer are incubated together with the HRP conjugate in a pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LDHB concentration since LDHB from samples and LDHB-HRP conjugate compete for the anti-LDHB antibody binding site. Since the number of sites is limited, as more sites are occupied by LDHB from the sample, fewer sites are left to bind the LDHB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Target Protein concentration in each sample is interpolated from the standard curve.
- 产品特点
- 针对L-Lactate Dehydrogenase B Chain (LDHB) BioAssay ELISA Kit (Rat) discontinued该产品的特点优势,欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到L-Lactate Dehydrogenase B Chain (LDHB) BioAssay ELISA Kit (Rat) discontinued产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品,为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译,仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前,请浏览说明书确认。
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