- 中文名称
Glucose Regulated Protein 94 (GRP94) BioAssay ELISA Kit (Rat) discontinued
- 英文名字
- Glucose Regulated Protein 94 (GRP94) BioAssay ELISA Kit (Rat) discontinued
- 供应商
- USBiological
- 产品货号
- 192996
- 产品报价
- ￥1/48Tests ￥1/96Tests
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- The BioAssay ELISA Kit is a 1.5 hour solid-phase Competitive ELISA designed for the quantitative determination of Glucose Regulated Protein 94 (GRP94) (Rat). This kit for research use only, not for therapeutic or diagnostic applications.Kit Detection Range:2.5-50ng/mLKit Sensitivity:0.1ng/mlAssay Principle:Glucose Regulated Protein 94 (GRP94) BioAssay ELISA Kit (Rat) utilizes the quantitative Competitive Enzyme Immunoassay technique, utilizing a monoclonal antibody and an conjugate to Glucose Regulated Protein 94 (GRP94) (Rat). The assay sample and buffer are incubated together with the HRP conjugate in a pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Target Protein concentration since Protein from samples and Protein-HRP conjugate compete for the anti-Protein antibody binding site. Since the number of sites is limited, as more sites are occupied by Protein from the sample, fewer sites are left to bind the Protein-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Target Protein concentration in each sample is interpolated from this standard curve.Materials and Equipment Supplied:192996A: Microtiter Strips, 1x489 wells or 1x96 wells192996B: Enzyme Conjugate, 1x3ml or 1x6ml192996C: Standard, 0ng/ml, 1x1 vial192996D: Standard, 2.5ng/ml, 1x1 vial192996E: Standard, 5ng/ml, 1x1 vial192996F: Standard, 10ng/ml, 1x1 vial192996G: Standard, 25ng/ml, 1x1 vial192996H: Standard, 50ng/ml, 1x1 vial192996J: Substrate A, 1x6ml192996K: Substrate B, 1x6ml192996L: Stop Solution, 1x6ml192996M: Wash Solution (100X), 1x10ml192996N: Balance Solution, 1x3mlMaterials and Equipment Required, but not Supplied:1. Precision pipettors and disposable tips to deliver 10-1000uL. A multi-channel pipette is desirable for large assays.2. 100mL and 1 Liter graduated cylinders.3. Distilled or deionized water.4. Tubes to prepare sample dilutions.5. Absorbent paper.6. Microplate reader capable of measuring absorbance at 450nm.7. Centrifuge capable of 3000×g.8. Microplate washer or washing bottle.9. Incubator (37°C).10. Data analysis and graphing software.4. Specimen Collection and Storage:Sample Type I: serum or plasmaSample Type II: cell culture supernatants, body fluid and tissue homogenatea. Serum:Use a serum separator tube and allow samples to clot for 2 hours at room temperature or overnight at 2-8°C. Centrifuge at approximately 1000 × g (or 3000rpm) for 15 minutes. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°Cb. Plasma:Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 1000×g (or 3000rpm) at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C.c. Tissue Homogenates:The preparation of tissue homogenates will vary depending upon tissue type. For this assay, tissues were rinsed in ice-cold PBS (0.02mol/L, pH 7.0-7.2) to remove excess blood thoroughly and weighed before homogenization. Minced the tissues to small pieces and homogenized them in a certain amount of PBS with a glass homogenizer on ice. The resulting suspension was subjected to ultrasonication or to two freeze-thaw cycles to further break the cell membranes. After that, the homogenates were centrifugated for 15 minutes at 1500×g (or 5000rpm). Remove the supernate and assay immediately or aliquot and store samples at -20°C or -80°C.d. Cell Lysates:Cells should be lysed according to the following directions-1. Adherent cells should be detached with trypsin and then collected by centrifugation. Suspension cells can be collected by centrifugation directly.2. Wash cells three times in PBS.3. Cells were resuspended in PBS and subjected to ultrasonication for 3 times. Alternatively, freeze cells at -20 °C. Thaw cells with gentle mixing. Repeat the freeze/thaw cycle for 3 times.4. Centrifuge at 1000×g (or 3000rpm) for 15 minutes at 2-8 °C to remove cellular debris.5. Assay immediately or store samples at -20°C or -80°C.e. Cell Culture Supernatants and other Body fluids:Centrifuge cell culture media at 1000×g (or 3000rpm) for 15 minutes to remove debris. Assay immediately or store samples at -20°C or -80°C. Simple Protocol:Please read Reagent Preparation above before starting assay procedure. It is recommended that all Standards and Samples be added in duplicate to the microtiter plate.1. Secure the desired numbers of coated wells in the holder then add 100uL of Standards or Samples to the appropriate well in the antibody pre-coated Microtiter Plate. Add 100uL of PBS (pH 7.0-7.2) in the blank control well.2. Dispense 10uL of Balance Solution into 100uL specimens, mix well.Note: This step is required when the sample is cell culture supernatants, body fluid and tissue homogenate; if the sample is serum or plasma, then this step should be skipped.3. Add 50uL of Conjugate to each well (NOT blank control well). Mix well. Mixing well in this step is important. Cover and incubate the plate for 1 hour at 37°C.4. Wash the microtiter plate using one of the specified methods indicated (manual or automatic).5. Add 50uL Substrate A and 50uL Substrate B to each well including blank control well, subsequently. Cover and incubate for 10-15 minutes at 20-25°C. (Avoid sunlight).6. Add 50uL of Stop Solution to each well including blank control well. Mix well.7. Determine the Optical Density (O.D.) at 450nm using a microplate reader immediately.Typical Certificate of Analysis:Important! Standard curve for demonstration only. Please refer to lot-specific Kit Protocol.1. Same Lot CV%: 4.4, 5.62. Different Lot CV%: 6.6, 7.93. Spike Recovery: 94-103%4. LinearityRange %1:296- 1011:493 - 1071:892 - 1001:1696 - 1085. Sensitivity: 1.0 pg/mL.6. Specificity: This assay has high sensitivity and excellent specificity for detection of the target antigen. No significant cross-reactivity or interference with analogues was observed. Note: Cross-reactivity detection between the target antigen and all analogues has not been determined and cross-reaction may exist in some cases.Contact:United States Biological4 Technology WaySalem, MA 01970www.usbio.netTech Support: tech@usbio.netCustomer Service: service@usbio.netTel: 781-639-5092 • 800-520-3011Fax: 978-594-8052
- 产品特点
- 针对Glucose Regulated Protein 94 (GRP94) BioAssay ELISA Kit (Rat) discontinued该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Glucose Regulated Protein 94 (GRP94) BioAssay ELISA Kit (Rat) discontinued产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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