- 中文名称
Bone Alkaline Phosphatase (BALP) BioAssay ELISA Kit (Rat) discontinued
- 英文名字
- Bone Alkaline Phosphatase (BALP) BioAssay ELISA Kit (Rat) discontinued
- 供应商
- USBiological
- 产品货号
- 183576
- 产品报价
- ￥1/48Test ￥1/96Tests
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- The BioAssay ELISA Kit is a 1.5 hour solid-phase competitive ELISA designed for the quantitative determination of Bone Alkaline Phosphatase (BALP) in rat serum, plasma, cell culture supernatant, tissue homogenates and body fluids. This kit is for research use only, not for therapeutic or diagnostic applications.Assay Principle:Bone Alkaline Phosphatase (BALP) BioAssay ELISA Kit (Rat) utilizes the quantitative Competitive Enzyme Immunoassay technique, utilizing a monoclonal antibody and a conjugate to Bone Alkaline Phosphatase (BALP) (Rat). The assay sample and buffer are incubated together with the HRP conjugate in a pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Target Protein concentration since Protein from samples and Protein-HRP conjugate compete for the anti-Protein antibody binding site. Since the number of sites is limited, as more sites are occupied by Protein from the sample, fewer sites are left to bind the Protein-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Target Protein concentration in each sample is interpolated from this standard curve.Detection Range:0.5-10ng/mlSensitivity:0.1ng/mlPrecision:Intra-Assay %CV: <10Inter-Assay %CV: <10Specificity:This assay has high sensitivity and excellent specificity for detection of Bone Alkaline Phosphatase (BALP). No significant crossreactivity or interference with analogs was observed. Note: Crossreactivity detection between BALP and all analogs has not been determined and crossreaction may exist in some cases.Kit Components:183576A: Microtiter Strips, 1x48 wells or 1x96 wells. Pre-coated with anti-BALP antibody.183576B: Enzyme Conjugate, 1x3ml or 1x6ml183576C: Standard, 0ng/ml, 1x1 vial183576D: Standard, 0.5ng/ml, 1x1 vial183576E: Standard, 1ng/ml, 1x1 vial183576F: Standard, 2.5ng/ml, 1x1 vial183576G: Standard, 5ng/ml, 1x1 vial183576H: Standard, 10ng/ml, 1x1 vial183576J: Substrate A, 1x6ml183576K: Substrate B, 1x6ml183576L: Stop Solution, 1x6ml183576M: Wash Solution, 100X, 1x10ml183576N: Balance Solution, 1x3mlNote: The BALANCE SOLUTION is used only when the sample is cell culture supernatants, body fluid and tissue homogenate; if the sample is serum or plasma, then the BALANCE SOLUTION is a superfluous reagentStorage and Stability:Store components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.Contact:United States Biological4 Technology WaySalem, MA 01970www.usbio.netTech Support: tech@usbio.netCustomer Service: service@usbio.netTel: 781-639-5092 • 800-520-3011Fax: 978-594-8052
- 产品特点
- 针对Bone Alkaline Phosphatase (BALP) BioAssay ELISA Kit (Rat) discontinued该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Bone Alkaline Phosphatase (BALP) BioAssay ELISA Kit (Rat) discontinued产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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