- 中文名称
AMPK Phosphorylation BioAssay Kit (5-AMP-activated Protein Kinase)
- 英文名字
- AMPK Phosphorylation BioAssay Kit (5-AMP-activated Protein Kinase)
- 供应商
- USBiological
- 产品货号
- 172364
- 产品报价
- ￥1/100Tests
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- The 5-AMP-activated protein kinase (AMPK) is a key sensor of intracellular energy balance. AMPK is activated in response to an increase in the AMP/ATP ratio which can be caused by a number of factors such as muscle contraction, starvation, or hypoxia. AMPK is a heterotrimeric protein complex comprising of alpha- (63kD), b- (38kD) and y- (38kD) subunits. For each subunit, isoforms have been identified (a1, a2, b1, b2, y1, y2, y3) which theoretically allow the formation of 12 different proteins. The alpha- subunit contains a serine/threonine kinase domain and the regulatory subunits contain binding sites for AMP and ATP (y-subunit) and for glycogen (b-subunit). AMPK is activated by phosphorylation on Thr-172 within the catalytic domain. AMP binding results in a 2 to 5-fold increase in AMPK activity compared to the basal level. Binding of AMP to the γ-subunit causes allosteric activation of the kinase and induces a conformational change in the kinase domain that protects AMPK from dephosphorylation of Thr-172. The cell-based ELISA measures phosphorylated AMPK in whole cells and normalizes the signal to the total protein content. The antibody recognizes both alpha-subunits and, thus, can be used for cells from all tissues (human, mouse, rat). This simple and efficient assay eliminates the need for cell lysate preparation and can be used to study AMPK regulation in short-term and long-term assays. In this assay, cells grown in 96-well plates are fixed and permeabilized in the wells. AMPK phosphorylation (pAMPK) is measured using a fluorescent ELISA followed by total protein measurement in each well.Intended Use:For quantitative fluorescent immunoenzymatic assay of AMPK phosphorylation status in cultured cells.Applications:Determination of AMPK phosphorylation status in whole cells. Evaluation of direct and indirect modulation of AMPK phosphorylation.Species tested: human, mouse, rat.Kit Components:10x Wash Buffer, 1x25mlProtein Stain, 1x6mlpAMPK-Ab1, 1x10ulBlocking Buffer, 1x25mlHRP Substrate, 1x6mlHRP-Ab2, 1x10ulStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对AMPK Phosphorylation BioAssay Kit (5-AMP-activated Protein Kinase)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到AMPK Phosphorylation BioAssay Kit (5-AMP-activated Protein Kinase)产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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