中文名称

ImmunoTek Plus™ TMB Microwell Peroxidase Substrate, Concentrate, 1L

英文名字

ImmunoTek Plus™ TMB Microwell Peroxidase Substrate, Concentrate, 1L

供应商

USBiological

产品货号

172015

产品报价

￥1/100ml

产品说明书

点击查看

购买方式

银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868

产品新闻

背景资料

USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！

产品描述

ImmunoTek Plus™ Detection Reagents and SubstratesTwo component ready to use TMB formulations for ELISA• Extremely sensitive• Superior quality and consistency• Exceptional stability for shelf life and shipping• Aqueous based and do not contain aprotic solvents• Reduces assay costsOverview of Product Advantages:• New products with unique, proprietary chemistry• Superior detection sensitivity• Competitive advantages in pricing• Superior signal-to-noise ratio• Superior lot-to-lot consistency• Increased stabilityTMB Microwell Peroxidase Substrate in an optimized buffer system, supplied as a two component ready to use solution.The substrate is supplied as two components; color solution (A) and stabilizer concentrate (B). The product is designed to be reconstituted with water to complete the manufacturing process and to produce a ready to use solution (TMB). The reconstituted product is a colorless liquid or very light yellow in appearance. When TMB is reacted with peroxidase, a reaction ensues producing a blue colored liquid product (oxidized state). A reagent (blue stop) tailored to TMB to stop the reaction at this “blue stage”. The reaction also can be terminated more typically using an appropriate reagent (an acid) or proprietary Yellow Stop specifically designed for TMB Substrates. By stopping the reaction in this manner, a second oxidized state is produced resulting in a soluble yellow reaction product. Depending on the stop reagent utilized, the colored product is stable for at least two hours. TMB is not recommended for membrane or immunohistochemistry applications where a colored precipitation reaction is desired. Product Use:The concentrated substrate is supplied as two components and designed to be reconstituted with water to complete the manufacturing process to produce a ready to use one component TMB solution. Equilibrate the product to RT (25°C) before use. Occasionally, due to the high concentration of solutes in component B, crystals may form during storage. Reconstitute component B with pure ASTM type 1 RODI water (9X the original component B volume). Gently agitate the resulting solution on occasion and equilibrate for at least 60 minutes or until all particulates are dissolved. Next, add Component A color solution to the reconstituted B stabilizer solution. Gently agitate the resulting solution on occasion and equilibrate for 60 minutes. Equilibrate this final solution overnight at room temperature and gently agitate a few times the next day. The final solution is now a completed product (TMBST).For immunoassay microwell applications, 100ul of substrate solution should be added to each well. A liquid soluble blue reaction develops which can be read at ~370nm or between 620nm and 650nm. Optimally, absorbance values should be monitored before they exceed 2.0OD units. In endpoint assays and for best results, the proprietary Yellow Stop reagent should be used. The substrate reaction also can be stopped using equal volumes of 1N HCL or 0.6N Sulfuric Acid. Addition of Yellow Stop or acid turns the blue color to yellow at end point. With Yellow Stop, absorbance values increase approximately three fold after addition. Hence, sample OD values should be monitored and the substrate reaction terminated when values reach ~0.7OD units. For Yellow Stop or an acid Yellow Stop, a liquid soluble yellow reaction develops which is read in the 450nm range. Dilution of the substrate is not recommended and will alter its performance characteristics. To reduce reaction kinetics, it is recommended that analytes, antibodies or conjugates used in the assay be diluted. TMB is light sensitive and should be protected from direct sunlight or UV sources. Storage and Stability:Store components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

产品形式

艾美捷向您提供的ImmunoTek Plus™ TMB Microwell Peroxidase Substrate, Concentrate, 1L产品形式查看产品说明书，建议按照产品说明书进行应用操作。

保存建议

接收到ImmunoTek Plus™ TMB Microwell Peroxidase Substrate, Concentrate, 1L产品后，置于4°C温度下保存。

其他

Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。

注意

该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。

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