- 中文名称
Sirt2 BioAssay Kit
- 英文名字
- Sirt2 BioAssay Kit
- 供应商
- USBiological
- 产品货号
- 171994
- 产品报价
- ￥1/1 Kit
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- The Fluorogenic SIRT2 BioAssay Kit is a complete assay system designed to measure Sirtuin 2 (SIRT2) activity for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 32 fluorescent SIRT2 activity measurements. In addition, the kit includes purified SIRT2 enzyme and a SIRT inhibitor, Nicotinamide, for use as a positive and negative control, respectively. The kit includes sufficient HDAC substrate 1, buffers, NAD+, nicotinamide, and detection reagents for a full 96-well plate. Researchers can test their own SIRT2-containing samples in the remaining wells, or additional SIRT2 enzyme may be ordered separately. The Fluorogenic SIRT2 Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only two simple steps on a microtiter plate are needed to analyze the SIRT2 activity level. First, the SIRT fluorometric substrate (SIRT substrate 1), containing an acetylated lysine side chain, is incubated with a sample containing purified SIRT2 enzyme. The deacetylation sensitizes the substrate so subsequent treatment with the Lysine Developer produces a fluorophore that can then be measured using a fluorescence reader. Applications:Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.Components: SIRT2 human recombinant enzyme, 1x135ugFluorogenic SIRT substrate 1, 1x50 ul (5mM)Nicotinamide Adenine Dinucleotide (NAD+) (50mM), 1x50ulNicotinamide (10mM), 1x500ul 2x SIRT Developer (contains 2mM Nicotinamide), 1x6ml HDAC assay buffer, 1x10mlblack, low binding NUNC black microtiter plate, 1 plate Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Sirt2 BioAssay Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Sirt2 BioAssay Kit产品后将其置于-20°C/-70°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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