The p300 Homogeneous BioAssay Kit contains an AlphaLISA® assay designed for screening inhibitors of p300 in a convenient 384-well format. This efficient method requires no time-consuming washing steps. Only a few simple steps on a microtiter plate are needed. The assay is based on the enzymatic transfer of an acetyl group from acetyl CoA to a specific lysine residue within a biotinylated peptide substrate. After the p300 reaction, the sample is incubated with a combination of an antibody that specifically binds the acetylated peptide and acceptor beads that bind the antibody. This is followed by incubation with streptavidin-labeled donor beads and reading of the Alpha-counts. Applications:Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.Components:P300 human recombinant enzyme, 1x1ugAcetyl CoA (1mM), 1x500ul3X HAT assay buffer, 1x4mlBiotinylated H3 Peptide Substrate, 1x700ulPrimary antibody 20, 1x40ul4X Detection Buffer, 1x2mlStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
